FastQCFastQC Report
Mon 22 May 2023
sRNA-ACR-150-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-150-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21526458
Sequences flagged as poor quality0
Sequence length15-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACCTCAGAGCAAAGATTTTCA3818221.7737335143570763No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC2679771.244872705021885No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGA2261651.0506373133935922No Hit
ACAAGTTCGGATCTACGGGA1960920.9109348133352919No Hit
TTCCGCCTCAGAAGAAAGATTTTCA1943620.9028981916114578No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTC1807150.8395017889148322No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGA1512030.702405384109174No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC1446550.6719870031567664No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC1235080.573749754836583No Hit
CCACAAGTTCGGATCTACGGGA1126580.5233466648345027No Hit
AGAATCATACCACTAGACCAAACAGCC1065150.4948096895457673No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTC1042070.4840879999858778No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT963840.44774667527746553Illumina RNA PCR Primer (100% over 50bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA928790.43146438675605625Illumina RNA PCR Primer (100% over 50bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGA750640.3487057647848987No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC739010.3433031109902056No Hit
AAGAGGTCAATGTGCGGGGAGGGGTG721140.3350016988396326No Hit
AGATTTCACTTGACTCAAATCCAGACAGA698510.32448905435348446No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC689230.32017808038832957No Hit
ACGACCAAAGTATAGAGCCGGTAGCGAA681170.3164338508453179No Hit
ACTTGTCAGTGGTAACTCAGGTCTTGGA594040.27595807912291004No Hit
TTCCGTCTCAGAAGAAAGATTTTCA582080.27040212560747334No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTA558550.2594713909738425No Hit
AGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTTG515180.23932409131125987No Hit
ACTCTTCATTTATCTACTCGTTAA494620.2297730541643219No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA489710.22749214013749963Illumina RNA PCR Primer (100% over 50bp)
ACATGAATGTTGTCAGTAGGTCAGATCAGA477770.22194547751422924No Hit
AGAGTGGATAACCCTCCCCAAGTCCGGACCTC463420.21527926238492184No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAA438740.20381430145172977Illumina RNA PCR Primer (100% over 50bp)
GCTCGTCAGTGGTAACTCAGGTCTTGGA424060.19699478660167874No Hit
TCATGGAAAATCGGCAGTTTT398220.18499095392284232No Hit
AGAATTCTACCACTGAACCACCAGTGC388000.18024330802587216No Hit
TCACAAGTTCGGATCTACGGGA366400.17020914448628752No Hit
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTC363970.16908030108808425No Hit
GCAAGTTCGGATCTACGGGA362430.16836490239128052No Hit
ACTCGTCGTATGTCCCTTAAATTCCATAA361090.16774241261613962No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC355290.16504805388791785No Hit
ACTGCTCGTCAGTAGTAACTCAGGCCTTGGA348280.16179159618363595No Hit
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATTCAGAGACTCTAGTCTAAAAGATTCATCATAAAA347220.16129917889882303No Hit
GCTTGGTAGACAGGTTCCGCACATAAAAGA334610.1554412713879822No Hit
AAGAGGTCAATGTGCGGGGAGGGGT321050.14914204649924293No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGA317870.1476647946448041No Hit
AAAATCCAGAGACTTCAAATCGTTAGCAC315190.1464198150945223No Hit
GAGAATTCTACCACTGAACCACCAGTGC306580.14242008601693784No Hit
AGGCTGATCCGAGGTCCG297440.13817414829694694No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC291590.13545656233830944No Hit
AGTGGATAACCCTCCCCAGGTCCGGACCTC284480.13215365017319616No Hit
TAAGAGTGGATAACCCTCCCCAAGTCCGGACCTC267270.12415883746411044No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGA262540.12196154146678474No Hit
AAGTGGATAACCCTCCCCAAGTCCGGACCTC258640.12014981749435973No Hit
TGGATAACCCTCCCCAAGTCCGGACCTC251040.11661927847117254No Hit
AGAGTCTCCAGATCAGGTCATTGTTACTA249110.11572270737712632No Hit
TTCATGGAAAATCGGCAGTTTT245500.11404570134111242No Hit
AGTGAATCCATGTACGATTTTCGATGTGA244270.11347431147288606No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAT243110.1129354397272417Illumina RNA PCR Primer (100% over 50bp)
CAGGCTGATCCGAGGTCCG240780.11185305078986986No Hit
ACTTGGTTGACAGGTTCCGCACATAAAAGA227260.10557240768546317No Hit
GATAACCCTCCCCAAGTCCGGACCTC224420.10425310099785111No Hit
AGGGTGATAACCCTCCCCAAGTCCGGACCTC223060.10362132033054393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)