FastQCFastQC Report
Mon 22 May 2023
sRNA-ACR-150-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-150-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21526458
Sequences flagged as poor quality0
Sequence length15-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAGGTGGAA3898731.8111340007724446No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2712501.2600772500520057No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT2307681.0720203017142904No Hit
TCCCGTAGATCCGAACTTGT1995550.9270219931212094No Hit
TGAAAATCTTTCTTCTGAGGCGGAA1981840.9206530865412229No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCCGTCTTCTGCTT1869350.8683964635519694RNA PCR Primer, Index 31 (100% over 42bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTC1819300.8451460059058484No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1549850.7199744611956134No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1442550.67012882472351No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCGCGTATGCCGTCTTCTGCTT1343860.6242829173289911RNA PCR Primer, Index 31 (97% over 42bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1271710.5907660238391286No Hit
TCCCGTAGATCCGAACTTGTGG1143130.5310348781021011No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1083280.5032318832945021No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT1062790.49371336427014606No Hit
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAG755110.35078227918406274No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT749290.3480786295636746No Hit
CACCCCTCCCCGCACATTGACCTCTT743190.3452449074529586No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA736060.341932704395679No Hit
TCTGTCTGGATTTGAGTCAAGTGAAATCT713680.3315361960616094No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT703080.3266120232134799No Hit
TTCGCTACCGGCTCTATACTTTGGTCGT694320.3225426124446483No Hit
TCCAAGACCTGAGTTACCACTGACAAGT604940.28102161535353376No Hit
TGAAAATCTTTCTTCTGAGACGGAA593520.27571651592658675No Hit
TAGTAACAATGACCTGATCTGGAGACTCC573190.26627232403956097No Hit
TTAACGAGTAGATAAATGAAGAGT504950.23457179996820657No Hit
TCTGATCTGACCTACTGACAACATTCATGT488230.22680461411719474No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCT463080.21512131721809505No Hit
TCCAAGACCTGAGTTACCACTGACGAGC437890.2034194385346628No Hit
AAAACTGCCGATTTTCCATGA410270.19058871645302725No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT396280.18408973738271295No Hit
TCCCGTAGATCCGAACTTGTGA374230.17384652876938697No Hit
TCCCGTAGATCCGAACTTGC373580.1735445747739828No Hit
TTATGGAATTTAAGGGACATACGACGAGT369200.171509869389567No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTT363160.16870401995534984No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA362930.16859717469543758No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT348070.16169404181589No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGC344260.15992412685821328No Hit
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAAGAAAACTTGATTCTGATT343920.15976618169138646No Hit
ACCCCTCCCCGCACATTGACCTCTT332850.15462367287734935No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG323590.15032198980436073No Hit
GTGCTAACGATTTGAAGTCTCTGGATTTT321080.14915598283749235No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC314380.14604353396178787No Hit
CGGACCTCGGATCAGCCT303920.14118439735882232No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT291680.13549837135305773No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT289710.1345832184746789No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT267300.12417277380235986No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTA267140.12409844666502962No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTT256030.11893735606665992No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA255400.11864469296342203No Hit
TAGTAACAATGACCTGATCTGGAGACTCT255220.11856107493392551No Hit
AAAACTGCCGATTTTCCATGAA253170.11760875848688158No Hit
TCACATCGAAAATCGTACATGGATTCACT250820.11651707865734345No Hit
CGGACCTCGGATCAGCCTG244900.11376697457612395No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT231940.10774647645237316No Hit
GAGGTCCGGACTTGGGGAGGGTTATC229580.10665015117675189No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACCCT222500.10336117534988803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)