FastQCFastQC Report
Mon 22 May 2023
sRNA-ACR-145-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-145-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20069848
Sequences flagged as poor quality0
Sequence length15-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAATTCTACCACTGAACCACCAGTGC4751172.367317380779366No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC4570472.27728182096845No Hit
TTCCGCCTCAGAAGAAAGATTTTCA3592141.7898192353026292No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC1768910.881376879386431No Hit
GAGAATTCTACCACTGAACCACAGTGC1689990.842054209877424No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC1485590.7402098909767528No Hit
AGAATCATACCACTAGACCAAACAGCC1408320.701709350265134No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGA1280310.6379271033841412No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1155820.5758987312709095Illumina RNA PCR Primer (100% over 50bp)
TGCGAGAATTCTACCACTGAACCACCAGTGC1051150.5237458699238778No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA1031690.5140497327134715Illumina RNA PCR Primer (100% over 50bp)
TTCCGCCCCAGAAGAAAGATTTTCA972460.48453780018662823No Hit
ACAAGTTCGGATCTACGGGA969380.48300315976483726No Hit
CCACAAGTTCGGATCTACGGGA953900.47529009686570617No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC909870.45335171447237665No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGA749520.373455743162579No Hit
AGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTTG674330.3359915829955463No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC609510.303694377755128No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATA605800.301845833610698No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA545620.27186055420051014Illumina RNA PCR Primer (100% over 50bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAA530080.26411759570874677Illumina RNA PCR Primer (100% over 50bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGC495200.2467382911918416No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGA448070.22325530317917705No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTA432070.21528314514389943No Hit
GCGAGAATTCTACCACTGAACCACCAGTGC403300.20094820847671593No Hit
ACTTGTCAGTGGTAACTCAGGTCTTGGA375920.18730585303884714No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC373870.18628442029057718No Hit
GCCGGATCCTAGCCACTAGACCATATGGGA364990.18185987258099812No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGA352480.17562664151716545No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTC347450.17312039433482507No Hit
AAGAGGTCAATGTGCGGGGAGGGGTG329960.16440582908251222No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGC300090.14952280655040337No Hit
TGGCGAGAATTCTACCACTGAACCACCAGTGC285350.14217845596040388No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAT283350.14118193620599417Illumina RNA PCR Primer (100% over 50bp)
TCACAAGTTCGGATCTACGGGA278730.13887997557330778No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTC273950.13649829336026859No Hit
AACCCTTTCTTTGAAATACAGCGTCTTTTA263850.1314658686004996No Hit
ACTCTTCATTTATCTACTCGTTAA261610.13034976647556076No Hit
GCCCAGTTCAATTGTAAAAGACTACAGTCA259020.12905927339360018No Hit
TTTCATTTGTATCCTCACGGTTGACCCCT249760.12444538693068329No Hit
AGGGACTCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTTG246380.12276126854573088No Hit
AGTGAATCCATGTACGATTTTCGATGTGA232460.11582549105503938No Hit
GAGGGACTCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTT230960.11507810123923211No Hit
GGAATCATACCACTAGACCAAACAGCC219050.10914382610172234No Hit
GAGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTT214540.10689667405552848No Hit
AGTTTTGGCTTAACAAGTCTGGAGGCTCTA208150.10371279344018947No Hit
GGGCGAGAATTCTACCACTGAACCACCAGTGC203100.10119658106030498No Hit
TGCTTGTCAGTGGTAACTCAGGTCTTGGA202960.10112682467749631No Hit
GAGAATCATACCACTAGACCAAACAGCC201520.10040933045432134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)