FastQCFastQC Report
Mon 22 May 2023
sRNA-ACR-145-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-145-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20069848
Sequences flagged as poor quality0
Sequence length15-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTC4852572.417840932327938No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4616512.3002217057149608No Hit
TGAAAATCTTTCTTCTGAGGCGGAA3666251.8267452748022808No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1762540.878202963968636No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC1723580.8587907591527351No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1523220.7589594101559712No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1434370.7146890200663203No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1313950.6546885656533123No Hit
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAG1261880.6287441738472559No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1046640.521498717877684No Hit
TGAAAATCTTTCTTCTGGGGCGGAA994700.4956190998556641No Hit
TCCCGTAGATCCGAACTTGT988060.4923106542710239No Hit
TCCCGTAGATCCGAACTTGTGG970710.48366584540151975No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT909130.45298300216324505No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT747140.37226988465483146No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT620460.3091503234105211No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA605330.3016116514684117No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCA499040.24865160912030823No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG454830.22662353994908183No Hit
TAGTAACAATGACCTGATCTGGAGACTCC444550.22150142841141596No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC418530.20853670640654576No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA381680.19017582993154705No Hit
TCCAAGACCTGAGTTACCACTGACAAGT381350.19001140417206946No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC376370.18753006998358931No Hit
TCCAAGACCTGAGTTACCACTGACGAGC363260.18099788299343372No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC349240.17401227951502174No Hit
CACCCCTCCCCGCACATTGACCTCTT340340.16957776660789856No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT299350.1491540942412718No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCA285290.1421485603677716No Hit
TCCCGTAGATCCGAACTTGTGA284790.14189943042916917No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT280150.13958750459893868No Hit
TAAAAGACGCTGTATTTCAAAGAAAGGGTT270040.13455009724039763No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGC267140.13310514359650358No Hit
TTAACGAGTAGATAAATGAAGAGT266920.1329955264235185No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA258210.12865568289306425No Hit
TCACATCGAAAATCGTACATGGATTCACT237950.118560937780894No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC224830.11202376819196638No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACT213380.10631869259797086No Hit
TCCAAGACCTGAGTTACCACTGACAAGCA209320.10429575749651915No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC205970.1026265869078829No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCC204980.10213330962945011No Hit
CGGACCTCGGATCAGCCT202160.10072821677573243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)