FastQCFastQC Report
Mon 22 May 2023
sRNA-ACR-140-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-140-S1-TP2_R2_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17691760
Sequences flagged as poor quality0
Sequence length15-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCCTCAGAAGAAAGATTTTCA2658401.5026204289454526No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGC2575691.4558698512753958No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGA1572710.8889505622956676No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGA1446620.8176800951403366No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGA1382070.7811941830547102No Hit
GGCGAGAATTCTACCACTGAACCACAGTGC1136290.6422707520337152No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGC1118610.6322773991960099No Hit
TTCCGCCCCAGAAGAAAGATTTTCA1055160.5964132454882951No Hit
TTCCGTCTCAGAAGAAAGATTTTCA840260.4749442678399436No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGC836510.47282463700615424No Hit
AGAATTCTACCACTGAACCACCAGTGC832600.47061456859012335No Hit
AGAATCATACCACTAGACCAAACAGCC712300.40261681144216294No Hit
AAGAGGTCAATGTGCGGGGAGGGGTG693070.39174734452649146No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGC654500.36994623485735734No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT640800.36220251687791377Illumina RNA PCR Primer (100% over 50bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGC523620.2959682925836661No Hit
AGCGAGAATTCTACCACTGAACCACAGTGC517850.2927068872740756No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA491160.27762076808638597Illumina RNA PCR Primer (100% over 50bp)
TTTTTTTGGCCTTTAACCTACAGCATTAATA484280.2737319520499939No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGA476440.26930051052015175No Hit
GAGAATTCTACCACTGAACCACCAGTGC462710.26153983549403786No Hit
AGGGACTCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTTG454010.25662229195964675No Hit
GAGGGACTCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTT427290.24151921572528678No Hit
ACTTGTCAGTGGTAACTCAGGTCTTGGA421410.23819563457790519No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTC417110.23576512455516013No Hit
AGAATTCTACCACTGAACCACAGTGC394910.22321691001912755No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGA394170.22279863620125978No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGC391460.22126684965204138No Hit
AGTGAATCCATGTACGATTTTCGATGTGA378490.21393575314157554No Hit
ACAAGTTCGGATCTACGGGA368300.20817600962255875No Hit
ACTTTGTAAAGTTTACATTGGTAGTTCAGA364610.20609029288211012No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTA362100.2046715533106938No Hit
ACTGCTCGTCAGTGGTAACTCAGGTCTTGGA353610.19987270910299484No Hit
CCACAAGTTCGGATCTACGGGA347730.19654912795561325No Hit
TGCGAGAATTCTACCACTGAACCACAGTGC312300.17652285583797203No Hit
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATTCAGAGACTCTAGTCTAAAAGATTCATCATAAAA287860.16270851515055598No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTC281410.1590627501164384No Hit
ACCGTGCAGAGTCTC268260.1516299113259506No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA260060.1469949852360647Illumina RNA PCR Primer (100% over 50bp)
GATAACCCTCCCCAAGTCCGGACCTC256250.14484144030893478No Hit
TGGCGAGAATTCTACCACTGAACCACCAGTGC247140.13969215047004935No Hit
GCGAGAATTCTACCACTGAACCACCAGTGC244740.13833558673642418No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAA243520.1376460001718314Illumina RNA PCR Primer (100% over 50bp)
TTTCATTTGTATCCTCACGGTTGACCCCT237630.1343167666755597No Hit
GCTTGGTAGACAGGTTCCGCACATAAAAGA235220.13295455059304442No Hit
ACTGTTCTCGCTGTTATTATATTCAACCAA231390.1307897009681343No Hit
CCAGGTCAACAGTCCGGAAATCATGTCAA225980.12773178021858764No Hit
AAGCTTCAAGAGCACTTAGACCAAAACCT221310.1250921332869087No Hit
GAGAATTCTACCACTGAACCACAGTGC218920.12374122190217368No Hit
GCTGCTTGTCAGTGGTAACTCAGGTCTTGGA217920.1231759870131632No Hit
ACTCTTCATTTATCTACTCGTTAA217790.12310250647759183No Hit
ATGAGGTCAATGTTCGGGGAGGGGTG212870.12032155082366029No Hit
ACTGCTCGTCAGTAGTAACTCAGGCCTTGGA212120.11989762465690242No Hit
AGAGTGGATAACCCTCCCCAAGTCCGGACCTC201480.11388352543783094No Hit
ACTCGTCGTATGTCCCTTAAATTCCATAA181800.10275970282210475No Hit
GGGCGAGAATTCTACCACTGAACCACCAGTGC179220.10130139680845772No Hit
AAGAGGTCAATGTGCGGGGAGGGGT178450.10086616594391964No Hit
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTC177870.10053832970829359No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)