FastQCFastQC Report
Mon 22 May 2023
sRNA-ACR-140-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-140-S1-TP2_R1_001.fastp-trim.20230522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17691760
Sequences flagged as poor quality0
Sequence length15-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAA2721351.538201965208662No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2606221.473126472436886No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT1608760.9093272800444953No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT1442840.815543507259877No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1422650.8041314148507555No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1170960.6618674456357084No Hit
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAG1161250.6563790148634167No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1114530.6299712408488471No Hit
TGAAAATCTTTCTTCTGGGGCGGAA1081600.6113580559537322No Hit
TGAAAATCTTTCTTCTGAGACGGAA859890.48603982871121926No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT854110.48277277105273864No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCT836650.4729037698906157No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT726490.4106374945172216No Hit
CACCCCTCCCCGCACATTGACCTCTT718220.40596300198510493No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA652290.36869706575264416No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT529320.2991901314510258No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCA527750.2983027126752793No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG485790.2745854567323997No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA485330.2743254486834549No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC475790.2689331078422949No Hit
TCCAAGACCTGAGTTACCACTGACAAGT429960.2430283928789448No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT427090.2414061687474847No Hit
TCCAAGACCTGAGTTACCACTGACGAGC407740.23046887364513197No Hit
GCACTGTGGTTCAGTGGTAGAATTCT404110.22841707099802394No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT391290.22117075972090963No Hit
TCACATCGAAAATCGTACATGGATTCACT390630.2207977046941627No Hit
TCCCGTAGATCCGAACTTGT376000.21252831826793941No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT375110.2120252592167201No Hit
TAGTAACAATGACCTGATCTGGAGACTCC373400.2110587075565122No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGT353910.20004227956969797No Hit
TCCCGTAGATCCGAACTTGTGG353890.2000309748719178No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA320610.18121995776564911No Hit
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAAGAAAACTTGATTCTGATT284650.16089411115683233No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC283520.1602553957322505No Hit
GAGACTCTGCACGGT273720.1547160938199478No Hit
GAGGTCCGGACTTGGGGAGGGTTATC262970.14863981876308519No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC253540.14330965375971638No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCA247270.1397656310056207No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA246760.13947736121222534No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGC242030.13680380018720578No Hit
TTGGTTGAATATAATAACAGCGAGAACAGT237000.1339606686954831No Hit
TTGACATGATTTCCGGACTGTTGACCTGG232440.13138319760159534No Hit
AGGTTTTGGTCTAAGTGCTCTTGAAGCTT226730.12815570638534549No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC223900.12655609164944584No Hit
CACCCCTCCCCGAACATTGACCTCAT222890.12598520441154526No Hit
TTAACGAGTAGATAAATGAAGAGT222600.1258212862937322No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGC219420.12402383934667892No Hit
AGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAGC215170.12162159106838437No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT212410.12006154277471547No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCT201590.11394570127562209No Hit
TTATGGAATTTAAGGGACATACGACGAGT185830.10503759942481698No Hit
ACCCCTCCCCGCACATTGACCTCTT185200.10468150144474038No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCC181680.10269187463542351No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTT177640.10040832568382117No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT177510.10033484514824981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)