FastQCFastQC Report
Mon 22 May 2023
sRNA-ACR-178-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-178-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17687362
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT5418943.06373556441034No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT3867832.1867760720903435No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2142371.2112433725277971No Hit
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC2017961.1409050145521984Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC1606670.9083717515364926No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG1566130.8854514313666448Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC1439050.8136035209772944No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1405950.7948895940502602No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG1248490.7058655779194206Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCAAGACCTGAGTTACCACTGACAAGTAGATCGGAAGAGCACACGTCTG852970.4822482855272595Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCCGTC771290.43606841992604667RNA PCR Primer, Index 33 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATG756140.4275029820727364TruSeq Adapter, Index 7 (97% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCGCGTATGCCGTC677440.38300793527039245RNA PCR Primer, Index 33 (97% over 34bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC624180.35289603955637927Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC598600.33843373590702786No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCGCGTATG585040.3307672449967383TruSeq Adapter, Index 7 (97% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG578950.32732410859233846No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT564960.31941450624462825No Hit
TTGACATGATTTCCGGACTGTTGACCTGGAGATCGGAAGAGCACACGTCT459170.2596034388847811Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG435870.24643019122919516Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTTTG369600.2089627610946166TruSeq Adapter, Index 7 (97% over 36bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT345820.19551813322981684No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCGCGTTTG292690.16547973632246574TruSeq Adapter, Index 7 (97% over 36bp)
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT290870.1644507530291968Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGTAGATCCGAACTTGCAGATCGGAAGAGCACACGTCTGAACTCCAG282810.1598938270161486Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTTTGCCGTC281880.15936802786079687RNA PCR Primer, Index 33 (97% over 34bp)
TCCAAGACCTGAGTTACCACTGACGAGTAGATCGGAAGAGCACACGTCTG274910.15542736107283833Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTTTATGTGCGGAACCTGTCTACCAAGCAGATCGGAAGAGCACACGTC273940.15487894690005213No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC273720.15475456430416248No Hit
TACTTCCTTCTTTGAGTTCTTTGTGCTTAGATCGGAAGAGCACACGTCTG263950.14923084629579017Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCGCGTTTGCCGTC261100.14761952630358333TruSeq Adapter, Index 7 (96% over 32bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC253160.14313044534283859No Hit
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAG247420.13988519034099037No Hit
TCACATCGAAAATCGTACATGGATTCACTAGATCGGAAGAGCACACGTCT246160.13917281729180417Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTGGTGGAAATTCTGAGCAGACTGCCAGATCGGAAGAGCACACGTCTG236180.13353037044190083Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGG233930.1322582757112112TruSeq Adapter, Index 7 (97% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG232820.1316307089774043Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT226560.12809145874890784No Hit
TTAAATGACGGGCTAAGAGTGGTATGATTAGATCGGAAGAGCACACGTCT221580.12527588907831477Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTCC221250.1250893151844803Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTGGTGGAAATTCTGAGCAGACTGCTAGATCGGAAGAGCACACGTCTG219590.12415079196094929Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCGCGGTTG212390.12008008882274247TruSeq Adapter, Index 7 (97% over 36bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTAGATCGGAAGAGCACACG207870.11752459185264597No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGTAGATCGGAAGAGCACACGTCT205740.1163203421742598Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC200050.11310335594420468No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGGTTG198440.11219310149246677TruSeq Adapter, Index 7 (97% over 36bp)
TAAGATTGTGGATTCCGTAAATAAAAAGTAGATCGGAAGAGCACACGTCT193030.10913442038445303Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCTAACTCGTATATATAAGCTTACAATAGATCGGAAGAGCACACGTC192110.10861427498345994No Hit
TCCAACGTAGTCTAGGTGGTTAGGATCTCCGGCTAGATCGGAAGAGCACA190570.10774359681223238No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGGATGCCGTC190200.10753440790096341RNA PCR Primer, Index 33 (97% over 34bp)
TAGAGCCTCCAGACTTGTTAAGCCAAAACTAGATCGGAAGAGCACACGTC189930.10738175653328065No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCGCGGATG183990.10402342644426003TruSeq Adapter, Index 7 (97% over 36bp)
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAA177920.10059159754857733No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)