FastQCFastQC Report
Mon 22 May 2023
sRNA-ACR-173-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-173-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19686271
Sequences flagged as poor quality0
Sequence length150
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT4206762.1369003809812432Illumina RNA PCR Primer (100% over 50bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2690861.366871359233041No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT2182141.1084577673445621Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG2130071.0820078622304854Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2118951.0763592556457238Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2008741.0203760783339821Illumina RNA PCR Primer (100% over 45bp)
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG1756310.8921496610505869Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1293170.6568892605410136No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC1188660.6038015020721802No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1173740.596222616258813No Hit
AGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTGA976540.49605128365854556Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT919640.4671478920512676Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT918220.4664265771816308No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA859320.4365072491382446No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT855920.4347801571968607Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA776140.394254452760505Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT720470.36597586206143357Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC637900.324032926296707No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT615540.31267475694101743Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC593210.3013318266318695No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT585370.29734935580232535No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT582970.2961302320789956Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTGAATCCATGTACGATTTTCGATGTGAGATCGTCGGACTGTAGAACTC510770.2594549267354899Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA503540.2557823165189588Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC495610.25175412854978985Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTTG486010.24687763365647056No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT481090.24437843002364437No Hit
CCAGGTCAACAGTCCGGAAATCATGTCAAGATCGTCGGACTGTAGAACTC420160.21342792649760842Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG419290.21298599414790137Illumina RNA PCR Primer (100% over 33bp)
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG389850.19803140980838882Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAAC374600.19028489448306385No Hit
GGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG369940.1879177625869318Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA342360.17390799913299984Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT333950.1696359864191649No Hit
GGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG328240.16673548789407602Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT320970.16304255894882275No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT308930.15692662160345147No Hit
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT292010.14833179935397617No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC277360.1408900649594837No Hit
ACTGCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC275280.13983349106593118No Hit
AGCCGAGATATCCGTCGCTGAAAGTTGTGGATCGTCGGACTGTAGAACTC265130.1346776136526821Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT260400.13227492398128626Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG237810.12079992193544425No Hit
ACTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT235230.11948936393286469Illumina DpnII expression Sequencing Primer (100% over 22bp)
ATTTCAGATTGTGCCGAAAGTATGTTCGTAGATCGTCGGACTGTAGAACT235040.1193928499714344No Hit
TGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTC230700.11718826790507965Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA219960.11173268924317865No Hit
ACTGCTCGTCAGTAGTAACTCAGGCCTTGGAGATCGTCGGACTGTAGAAC213540.10847153328327136No Hit
GCTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC212770.10808039775536972No Hit
GGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA209390.10636346517834687No Hit
AGTTTTGGCTTAACAAGTCTGGAGGCTCTAGATCGTCGGACTGTAGAACT206320.10480400274892081No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG202540.10288388288467634No Hit
TTTCATTTGTATCCTCACGGTTGACCCCTGATCGTCGGACTGTAGAACTC202050.10263497845782983Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACTCT198900.10103487857095943Illumina DpnII expression Sequencing Primer (100% over 22bp)
ATTGTAAGCTTATATATACGAGTTAGATCAGATCGTCGGACTGTAGAACT197510.10032880274786424No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)