FastQCFastQC Report
Mon 22 May 2023
sRNA-ACR-150-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-150-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22586680
Sequences flagged as poor quality0
Sequence length150
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT5424812.401773965894943Illumina RNA PCR Primer (100% over 50bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA3719521.6467758873814125Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2969641.3147749027302817Illumina RNA PCR Primer (100% over 45bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2535221.1224403055252032No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT2233990.989074091455672No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT1900980.8416376377581832Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1883430.8338675715067464Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC1718990.7610636003166469No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT1480030.655266732428139Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1361840.6029394315587772No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1205880.5338898855431609No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT1091190.48311217053590877Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG1039330.4601517354476178Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT1021340.452186864116373No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC714180.3161952088576099No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC697280.3087129228377079No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA692670.3066718968879003Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGATTTCACTTGACTCAAATCCAGACAGAGATCGTCGGACTGTAGAACTC686540.3039579079351193Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT671830.29744521992608036No Hit
ACGACCAAAGTATAGAGCCGGTAGCGAAGATCGTCGGACTGTAGAACTCT668280.29587349712308314Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT580690.257094004076739Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA560510.2481595347346312Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC547310.24231538234038824Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTTG516270.2285727694375623No Hit
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG489980.2169331659190284Illumina RNA PCR Primer (100% over 33bp)
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC479990.21251020512974902Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACATGAATGTTGTCAGTAGGTCAGATCAGAGATCGTCGGACTGTAGAACT469750.20797655963603326No Hit
AGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAA439940.19477851547903455No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT415100.18378088324623185Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCATGGAAAATCGGCAGTTTTGATCGTCGGACTGTAGAACTCTGAACGTG383650.16985674742813023Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG377010.1669169616782989Illumina DpnII expression Sequencing Primer (100% over 23bp)
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATTCAGAGACTCTAGTCT372580.16495562871568553No Hit
ACTCGTCGTATGTCCCTTAAATTCCATAAGATCGTCGGACTGTAGAACTC355240.15727853761597543Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT354240.15683579879822976Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT351420.15558727533218694Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT347210.15372334490947762No Hit
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA346620.15346212900700767No Hit
ACTGCTCGTCAGTAGTAACTCAGGCCTTGGAGATCGTCGGACTGTAGAAC332140.14705127092605022No Hit
GCTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT330360.1462631958304629No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT312380.13830275188739558No Hit
AAAATCCAGAGACTTCAAATCGTTAGCACGATCGTCGGACTGTAGAACTC307970.13635027370113714Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGAGGTCAATGTGCGGGGAGGGGTGATCGTCGGACTGTAGAACTCTGAA306710.13579242279077758Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT297320.13163510529214564Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGGCTGATCCGAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG287920.12747336040533624Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTGGATAACCCTCCCCAGGTCCGGACCTCGATCGTCGGACTGTAGAACT279150.12359054097370664No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA276830.12256338691653665No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT259610.11493942447495605No Hit
TAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG255290.1130267927822947No Hit
AAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC245930.10888275744819513No Hit
TGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCT245510.10869680714474193Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC244260.10814338362255985Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTGAATCCATGTACGATTTTCGATGTGAGATCGTCGGACTGTAGAACTC239660.10610678506092971Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTCATGGAAAATCGGCAGTTTTGATCGTCGGACTGTAGAACTCTGAACGT236780.10483169726582216Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCTGATCCGAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTA232840.10308730632390417Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG231580.10252945541354462No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)