FastQCFastQC Report
Mon 22 May 2023
sRNA-ACR-150-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-150-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22586680
Sequences flagged as poor quality0
Sequence length150
%GC66

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC3801861.6832310016345917Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCCGTC2985811.3219339894132294RNA PCR Primer, Index 31 (100% over 34bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2634421.1663599962455748No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC2282841.0107018827025485No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCGCGTATGCCGTC2135790.9455971395530463RNA PCR Primer, Index 31 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATG1957750.8667719204416054TruSeq Adapter, Index 7 (97% over 35bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG1936250.8572530358600733Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC1935370.856863425700457Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT1768660.7830544373940749No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG1514460.6705102299231228Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1414100.6260769621741663No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1251230.5539680909279274No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCGCGTATG1158890.5130855884972914TruSeq Adapter, Index 7 (97% over 35bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC1106500.4898905018355952Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA1058330.4685637729847857Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC1050150.46494216945562605No Hit
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAG758440.3357908289310337No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT731460.32384573562825525No Hit
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA728890.32270789686664886Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT724530.3207775556212777No Hit
TCTGTCTGGATTTGAGTCAAGTGAAATCTAGATCGGAAGAGCACACGTCT703580.3115021773895057Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC696230.30824804707907494No Hit
TTCGCTACCGGCTCTATACTTTGGTCGTAGATCGGAAGAGCACACGTCTG678960.30060194769660703Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAAGACCTGAGTTACCACTGACAAGTAGATCGGAAGAGCACACGTCTG597300.26444789583949474Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC579400.2565228710018471Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT564090.24974453970216076Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACG498310.2206211802708499TruSeq Adapter, Index 7 (97% over 35bp)
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT490430.21713239838701393Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGATCTGACCTACTGACAACATTCATGTAGATCGGAAGAGCACACGTC482860.21378086553667913No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTAGATCGGAAGAGCACACG452970.20054740227426074No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG429080.18997037191831645Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAA403870.17880892632294787No Hit
AAAACTGCCGATTTTCCATGAAGATCGGAAGAGCACACGTCTGAACTCCA396130.1753821278735963Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA388250.17189334598976036Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTCC367090.16252499260626174Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCGTAGATCCGAACTTGCAGATCGGAAGAGCACACGTCTGAACTCCAG365100.16164394235894786Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTATGGAATTTAAGGGACATACGACGAGTAGATCGGAAGAGCACACGTCT365000.16159966847717327Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC359810.1593018540130732No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAGATCGGAAGAGCACAC356740.15794264584259393No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGCAGATCGGAAGAGCACACGTC341240.15108019416753593No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGTAGATCGGAAGAGCACACGT340410.150712720948807No Hit
ACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAAC325730.1442133151043004Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC318210.1408839191948529No Hit
GTGCTAACGATTTGAAGTCTCTGGATTTTAGATCGGAAGAGCACACGTCT317250.14045888992981703Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG306210.13557105338190473Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGACCTCGGATCAGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC294350.13032017100344095Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGGTCCGGACCTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC286490.1268402438959599No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG285140.12624254649200325No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC264170.11695831348387632No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAGATCGGAAGAGCACA261820.115917877262174No Hit
TAGTAACAATGACCTGATCTGGAGACTCTAGATCGGAAGAGCACACGTCT252650.1118579623034461Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTTAGATCGGAAGAGCACACGT251180.11120713624135994No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCAAGATCGGAAGAGCACACGTCTG250380.1108529451871634Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACATCGAAAATCGTACATGGATTCACTAGATCGGAAGAGCACACGTCT247420.10954243828663619Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAACTGCCGATTTTCCATGAAAGATCGGAAGAGCACACGTCTGAACTCC244650.10831605176148065Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACCTCGGATCAGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT236150.10455277181064238Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTTTTATGTGCGGAACCTGTCAACCAAGTAGATCGGAAGAGCACACGTC228640.10122780328937232No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)