FastQCFastQC Report
Mon 22 May 2023
sRNA-ACR-145-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-ACR-145-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21295979
Sequences flagged as poor quality0
Sequence length150
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT6449883.028684429112181Illumina RNA PCR Primer (100% over 50bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT4633012.1755327613724638Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC4333192.0347456202882244No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA3483551.6357782847175046Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2712851.2738789796890766Illumina RNA PCR Primer (100% over 45bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1671460.7848711721588381No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG1645020.7724556828310171Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1451570.6816169381083631No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG1371910.6442108155722731Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT1253190.588463202372617Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC993710.46661860438536307No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA942560.44259998565926456Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT938750.4408109155254144Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT922550.43320384566494924Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA865150.40625040060379475No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC712430.33453733214143383No Hit
AGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTTG675390.3171443773493578No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT594140.27899163499362956No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC577060.27097134158518843No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG478080.22449308388217326No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT440830.20700151892523935No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC423450.19884035385271556Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT395300.18562189603962326No Hit
ACTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT366890.1722813494509926Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT366160.17193856173505806No Hit
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT357580.16790963214229315No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT344100.16157979870284433Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG334420.15703433967510955Illumina RNA PCR Primer (100% over 33bp)
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC329560.15475221871697No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA318110.14937561687114737Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG305100.14326648237209474No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAAC284450.13356981616106967No Hit
TGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA271000.1272540698880291No Hit
TCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT269910.12674223617519534Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT268390.1260284864105097No Hit
AACCCTTTCTTTGAAATACAGCGTCTTTTAGATCGTCGGACTGTAGAACT258450.1213609386072366No Hit
GCCCAGTTCAATTGTAAAAGACTACAGTCAGATCGTCGGACTGTAGAACT255880.12015413801826157No Hit
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC254020.11928073370094891Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGGACTCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTTG246560.11577772498742601No Hit
TTTCATTTGTATCCTCACGGTTGACCCCTGATCGTCGGACTGTAGAACTC242920.1140684821298894Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGGGACTCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTT231090.10851344284289538No Hit
AGTGAATCCATGTACGATTTTCGATGTGAGATCGTCGGACTGTAGAACTC228280.10719394492265419Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGGGACGCTCGGACAGCCTTGCTCGCCTCGCAAGAGACGAGCGCCATTT214810.10086880720534144No Hit
GGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG213910.10044619221309338Illumina DpnII expression Sequencing Primer (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)