FastQCFastQC Report
Fri 19 May 2023
RNA-POC-57-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POC-57-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42513488
Sequences flagged as poor quality0
Sequence length15-130
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCAC792600.18643494977405758No Hit
TTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTT665540.15654796426018963No Hit
TCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCAGTGTTA641220.15082742681569672No Hit
GTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAA632300.14872926916746987No Hit
ATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCACGCT571520.1344326299455834No Hit
TGTATAACTTTAATGGATGAACAAACCAACCCTTACGAGTGACTACACCG559350.13157000902866403No Hit
GTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCACGCTTTTAACTC508980.1197220044612665No Hit
TATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCACGCTT507570.1193903450123876No Hit
TAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTA477160.11223732101209856No Hit
ATGTATAACTTTAATGGATGAACAAACCAACCCTTACGAGTGACTACACC465180.10941939179396432No Hit
TTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAAA435020.10232517266049777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)