FastQCFastQC Report
Fri 19 May 2023
RNA-POC-48-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POC-48-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51579450
Sequences flagged as poor quality0
Sequence length15-130
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAACGTTCGAGTTGGCTATCGGTAGTGCAACGGGGGCTCTTGTCCCCCA1224710.23744146166738886No Hit
TAAGTTCGCCGTTCGTCGCCTCCCGATCTGACCCGAGGGTCACAGCTGGG792620.15366972699398695No Hit
GTTCGCCGTTCGTCGCCTCCCGATCTGACCCGAGGGTCACAGCTGGGCCA706100.13689560474181092No Hit
AACATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCAC654350.12686253924770427No Hit
ACAAAGTAAACATTGAAGGGAGATGGAAGAAGGAACTCTAGCCAGAGTCT630310.12220176833991057No Hit
CCCCCACCGCCCCAAGGATCTCCCGGTCCCCGCCCGGCGTGCTGACGTCA613150.11887486198476332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)