FastQCFastQC Report
Fri 19 May 2023
RNA-POC-47-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POC-47-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54219233
Sequences flagged as poor quality0
Sequence length15-130
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAACGTTCGAGTTGGCTATCGGTAGTGCAACGGGGGCTCTTGTCCCCCA1079950.19918208728625875No Hit
AACATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCAC899040.16581569864700965No Hit
TAAGTTCGCCGTTCGTCGCCTCCCGATCTGACCCGAGGGTCACAGCTGGG874720.1613302054641754No Hit
GTTCGCCGTTCGTCGCCTCCCGATCTGACCCGAGGGTCACAGCTGGGCCA691970.12762445385385662No Hit
GTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAA676430.12475831223949627No Hit
ATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCACGCT594930.10972674585787666No Hit
TTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTT559780.10324380649206158No Hit
TGTATAACTTTAATGGATGAACAAACCAACCCTTACGAGTGACTACACCG549090.10127218140470559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)