FastQCFastQC Report
Fri 19 May 2023
RNA-POC-57-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POC-57-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42934866
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAATAATTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAA754530.1757382915786904No Hit
GTTTTTTAAAAAGTAACGAAAATGAGCTATGACGCATGTTTAACTTTGAA542370.12632390654252887No Hit
CAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCGATGGT449380.10466551822940358No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)