FastQCFastQC Report
Fri 19 May 2023
RNA-POC-57-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POC-57-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42934866
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTTTCGTACGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAG661300.15402400463995858No Hit
GGGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTA633000.14743262503719004No Hit
CGGGATACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGCTA601460.14008661398873354No Hit
CTTTCGTACGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAGAAA574670.133846929905406No Hit
CCCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAAC568190.13233766701403005No Hit
CACGCTTTTAACTCAAATCATGTATAACTTTAATGGATGAACAAACCAAC556410.12959397614051013No Hit
CGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTG512840.11944604648352693No Hit
TTTCGTACGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAGAAAC509340.11863085819343189No Hit
GTCGAAGTTTAACTTTGCCGAGTTCCTTAAAAAAATGTTTTTCTTTTTTT478290.11139897350558867No Hit
GGGACTTCTCGTCTAACAATTAAAATGTAGCATCTTCACTACAGATTTAA458980.10690146325366429No Hit
CCACGCTTTTAACTCAAATCATGTATAACTTTAATGGATGAACAAACCAA457750.10661498279743088No Hit
CTCATTTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTA448690.10450480968078485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)