FastQCFastQC Report
Fri 19 May 2023
RNA-POC-48-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POC-48-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52036501
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAAAGGATTTATCTCCGGAAACGTTCGAGTTGGCTATCGGTAGTGCA1232800.23691062548575276No Hit
CCCCTAGTCACTGCCAGTTCTAAGTTCGCCGTTCGTCGCCTCCCGATCTG696480.13384451041394962No Hit
CTAGTCACTGCCAGTTCTAAGTTCGCCGTTCGTCGCCTCCCGATCTGACC676480.13000105445214313No Hit
GGGACTCTGTAGGGACCAGAACAAAGTAAACATTGAAGGGAGATGGAAGA647880.12450491242675982No Hit
CGCCGGGAACTGCCGCTGGCCCCCCACCGCCCCAAGGATCTCCCGGTCCC623680.11985433071297397No Hit
ATCCTTTCGTACGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAG548940.10549133578370307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)