FastQCFastQC Report
Fri 19 May 2023
RNA-POC-47-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POC-47-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54705696
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAAAGGATTTATCTCCGGAAACGTTCGAGTTGGCTATCGGTAGTGCA1087850.19885497846513092No Hit
CCCCTAGTCACTGCCAGTTCTAAGTTCGCCGTTCGTCGCCTCCCGATCTG780770.1427218840246544No Hit
ATCCTTTCGTACGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAG763260.13952112043323606No Hit
CTAGTCACTGCCAGTTCTAAGTTCGCCGTTCGTCGCCTCCCGATCTGACC666490.12183192039088579No Hit
CCCCAGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAAC610030.11151124007269736No Hit
CTTTCGTACGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATAGAAA598970.10948951275567356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)