FastQCFastQC Report
Fri 19 May 2023
RNA-POR-82-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-82-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48908730
Sequences flagged as poor quality0
Sequence length15-130
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAAATGTGTACAGAAGGCTGAAAGGAGAGAACTTTAGTCTTCTTGTTT1783540.36466700321190104No Hit
TTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGACATGG1478170.30223029712691374No Hit
TTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGC1367210.2795431408666714No Hit
AGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCG1324050.2707185404323523No Hit
ACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAA1263950.25843034566630535No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG967410.19779904323829306No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA798450.16325306341015192No Hit
GGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCAC777080.15888370031280713No Hit
TTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGATGGCCCG709530.14507226010571117No Hit
TGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAA693970.14189082398990938No Hit
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAA692920.14167613839083534No Hit
ATTTCCACTGGGCGCCTCGGAGCTGCGGACTTCCCAGTGTGCATCGGGGC686100.14028170430923068No Hit
TTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAG675470.13810826819670027No Hit
TGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAG623090.12739852374003577No Hit
ATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGTTGGAT600320.12274291317725895No Hit
CCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTG600140.1227061099317034No Hit
ATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCA598770.12242599634053061No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGC594090.12146911195608638No Hit
TAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGA583240.11925069409898804No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG579190.11842262107398821No Hit
GAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCGCAGAT576080.11778674277577847No Hit
TTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTG571040.11675625190022312No Hit
GTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCT544010.11122963119263166No Hit
CCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGA537170.10983110786152084No Hit
TGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTAT523870.10711175693991644No Hit
GCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAATTGTAATTGCGAG523340.1070033918280029No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC508360.10394054394788005No Hit
GCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG497620.10174461696306569No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)