FastQCFastQC Report
Fri 19 May 2023
RNA-POR-82-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-82-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48908730
Sequences flagged as poor quality0
Sequence length15-130
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGG2397410.4901803829295915No Hit
GGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAA1984420.40573942525189266No Hit
GCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCT1926090.393813129067142No Hit
ACAAAGTAAACATTGAAGGGAGATGGAAGAAGGAACTCTAGCCAGAGTCT1808020.36967224460745557No Hit
GTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCA1784230.36480808231986395No Hit
TGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCA1284710.2626749866537119No Hit
ATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCAT1279540.26161791565636644No Hit
AGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCCCTGTA1112550.2274747269045833No Hit
CCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGA1027920.21017106761921645No Hit
TGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCT1022500.20906288100304382No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC1006860.20586508788921734No Hit
TTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTA867730.17741822369953178No Hit
TGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGG815720.16678413035873146No Hit
CGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATC800220.16361496199144815No Hit
ACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCCAT785830.1606727469717574No Hit
TTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGGTTATG783660.16022906340033774No Hit
CATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCA782240.15993872668539952No Hit
TCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCCACGGG776000.15866288083947386No Hit
CGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCCTC768690.157168260144968No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC716930.14658528242299484No Hit
TTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAA684780.14001181384182335No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG682270.13949861302879873No Hit
TTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGT678370.1387012093750952No Hit
CCCCCACCGCCCCAAGGATCTCCCGGTCCCCGCCCGGCGTGCTGACGTCA677210.13846403290373724No Hit
AGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATT673520.1377095663698485No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC639720.1307987347044178No Hit
TCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACAGA632110.12924277526731937No Hit
ACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCA605450.12379180567559207No Hit
AAGATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGT554640.11340306730516209No Hit
GCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCC550340.1125238786613351No Hit
GAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTATGCAAAGTCCGT537540.10990675897738503No Hit
AGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGG536190.10963073463571842No Hit
GCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGAAT523270.1069890794547313No Hit
TATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCT518100.10593200845738582No Hit
TAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAG504810.10321470216053454No Hit
AGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCC495990.10141134312831267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)