FastQCFastQC Report
Fri 19 May 2023
RNA-POR-79-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-79-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49976568
Sequences flagged as poor quality0
Sequence length15-130
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGACATGG2748370.5499317200012614No Hit
AGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCG2404450.48111546995383914No Hit
TTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGC2384180.4770595691965082No Hit
ACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAA2202890.44078456928054766No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG1686880.33753418201906144No Hit
GGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCAC1401310.28039340356464654No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA1333160.2667570130065754No Hit
TGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAA1302030.2605280938859187No Hit
TTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAG1248590.24983508271316268No Hit
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAA1198510.23981438661414287No Hit
TTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGATGGCCCG1188560.23782345358328727No Hit
CCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTG1175810.23527225799098492No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGC1136940.2274946130754717No Hit
TGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAG1115450.22319459791636753No Hit
ATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGTTGGAT1115300.22316458385057575No Hit
ATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCA1097810.21966494377925272No Hit
TTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTG1085070.21711574912466977No Hit
TAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGA1060400.21217943577077963No Hit
CCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGA1051110.21032056462940793No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG1033370.2067709011150986No Hit
GAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCGCAGAT1016610.2034173294972956No Hit
TGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTAT979490.19598984868268665No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC934030.18689358581005402No Hit
CAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGACA905280.1811408898666271No Hit
GGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAACGGCC903930.18087076327450097No Hit
CTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGCCGAGG893860.17885581899101194No Hit
GTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCT881500.17638265996976824No Hit
ACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTGTAGAATAAGTGGGA851640.17040785993948204No Hit
GATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAATGGAC845680.16921530105868815No Hit
GCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAATTGTAATTGCGAG844470.16897318759463437No Hit
CGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGATT833970.16687220298920888No Hit
GCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG818540.16378475608809312No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC805400.1611555239247321No Hit
AACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGATCCT804590.16099344796945642No Hit
AAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGAA783250.15672344687614403No Hit
AAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAG780660.15620520400680576No Hit
GGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG773420.15475652509792187No Hit
TGATTAGAGGCATTAGGGTTAAAACAACCTTAACCTATTCTCAAACTTTA758550.15178113070909552No Hit
GCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCG741830.14843556284217035No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA710790.14222465216098873No Hit
GTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGACAGCAG705660.14119817111090943No Hit
GTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTT703540.1407739723143854No Hit
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC692820.13862896707913197No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT655100.13108143000135583No Hit
AAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGC649630.12998691706881513No Hit
TTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAACCTTAA646190.12929859449332334No Hit
TTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATG640690.1281980787476243No Hit
ATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGC637870.12763381431073859No Hit
GGTGGTAAACTCCATCTAAAGCTAAATACTGACGTGAGACCGATAGCGAA634580.1269755058010386No Hit
GGATTGTCCGGAGATAGGGTTTAATGCCTGGTAAAGCACCACACTTCTTG628700.12579895442200031No Hit
TCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCA617930.12364394449814961No Hit
CCACACTTCTTGTGGTGTCCGGTGCGTTCTCGACGGCCCTTGAAAATCCG617040.1234658610411183No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAACTCAGTGTACA612940.12264547657614264No Hit
GTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGACGTGCA594350.11892573335567981No Hit
AAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAG594210.11889772022760747No Hit
AGGGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAACGG586400.1173349878687148No Hit
GTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATGGCGCT582730.1166006437256756No Hit
TGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGAACGCG562790.11261077391308662No Hit
AGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCAT555370.11112607812525262No Hit
GCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGA555050.11106204811823013No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAA549310.10991350986726418No Hit
GTTAAAACAACCTTAACCTATTCTCAAACTTTAAATTGGTAAGATGTCCG547710.10959335983215174No Hit
ATCGTTGACCGACCTATTCTACTCTTAGGAAGGTTTGAGTAAGAGCGTGT539140.10787855620658066No Hit
GATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGG538990.10784854214078886No Hit
GCCGGACGACGAATGAGAGTGCCTAGTGGGCCATTTTTGGTAAGCAGAAC535480.10714621300126093No Hit
AAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTA531780.10640586604506336No Hit
GGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGA529520.105953654120467No Hit
TGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAAT528390.10572754815816884No Hit
CTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGACAT517760.10360055136239046No Hit
ACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCGAAAAGTCC517550.10355853167028196No Hit
GTCTGACCTTGTGAAAAGACATGAGAGGTGTAGAATAAGTGGGAGCAGCA516880.10342446884307863No Hit
GGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGACCTTGTGAAAAGA514630.10297425785620172No Hit
TGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGC513580.10276415939565918No Hit
GATAGGGTTTAATGCCTGGTAAAGCACCACACTTCTTGTGGTGTCCGGTG509730.10199379837366983No Hit
GTGTACAGTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAA509140.10187574304822211No Hit
CCGAGGTGAGCAGCCTCTGGTTGATAGAACAATGTAGGTAAGGGAAGTCG507910.10162962770872942No Hit
CTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAATTGTAATTGCGAGA503590.10076522261392579No Hit
CGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGAT503050.10065717197707534No Hit
TTCTCAAACTTTAAATTGGTAAGATGTCCGACTTGCTTAATTGAAGCCGG502430.10053311383846925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)