FastQCFastQC Report
Fri 19 May 2023
RNA-POR-79-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-79-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49976568
Sequences flagged as poor quality0
Sequence length15-130
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGG4723220.9450869055274064No Hit
GGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAA3286180.6575441514911547No Hit
GTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCA3278860.6560794650805154No Hit
GCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCT3168880.6340731520419729No Hit
TGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCA2410700.48236605602849714No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC2095770.4193505244297687No Hit
CCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGA1981560.39649781473589785No Hit
ATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCAT1943160.3888142138931989No Hit
AGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCCCTGTA1868130.3738011781841442No Hit
TGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCT1803050.3607790755059451No Hit
TCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACAGA1748410.34984595180685474No Hit
TGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGG1627250.32560259039796413No Hit
TTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGGTTATG1482200.2965789887773006No Hit
TTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTA1452540.29064420750140346No Hit
ACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCCAT1434470.28702851304235216No Hit
TCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCCACGGG1413600.2828525560218541No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC1392310.27859255961713897No Hit
CGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATC1383430.27681572692226486No Hit
CGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCCTC1359300.2719874642052251No Hit
CATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCA1358330.2717933732464382No Hit
AGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATT1346040.2693342207892307No Hit
TTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGT1322380.26460000214500523No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG1263870.25289251554848663No Hit
TTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAA1191340.23837971426929516No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC1071040.21430843350427742No Hit
TATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCT1068930.2138862356454729No Hit
AAGATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGT1031030.2063026816887466No Hit
AGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGG1017120.2035193773209877No Hit
GCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCC1003340.20076208514358168No Hit
ACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCA1000210.20013579163739295No Hit
GAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTATGCAAAGTCCGT982690.19663014875291157No Hit
GCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGAAT955900.1912696366024974No Hit
AGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCC954580.19100551282352962No Hit
ACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCG926360.18535886657923367No Hit
ATTAACCCGATTCCCTTTCGATAGACGGCCCAAGATTGGGCACTTTGACA883750.17683287095664513No Hit
TGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGG877040.17549024174689226No Hit
TTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACA874270.17493598199860383No Hit
TAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAG873290.17473989010209745No Hit
CTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGG871720.17442574288014334No Hit
GACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCACTTCAT864530.17298706865985675No Hit
AGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCT856060.17129227441148018No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA836840.16744647211469182No Hit
TCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGG820830.16424297082584782No Hit
AGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGC818200.16371672420563171No Hit
CTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACAGAT814660.16300839225294542No Hit
GCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCG792430.15856030770260174No Hit
ACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCT785090.15709161941652336No Hit
CAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATTCCC780100.15609315149451639No Hit
AAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTAGTTGGCAT759160.15190318790998214No Hit
ATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTT749850.15004031489317154No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC743980.1488657644518527No Hit
ATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCAC743700.14880973819570803No Hit
GACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCCA742870.14864366036499346No Hit
TTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAGCACA740050.14807939592810776No Hit
GGACACGAAAATCATTGTCTCCCGCGGATTTTCAAGGGCCGTCGAGAACG737060.14748111554999135No Hit
GCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCTC732540.14657669170079865No Hit
ATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGT728460.1457603091112619No Hit
CCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTC725100.14508799403752576No Hit
GGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGT720820.14423159269359992No Hit
GGAGTTTACCACCATTTTTGGGCTGCATTCCCGAACAACCCGACTCTTCG707140.14149430989338843No Hit
TGACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCC706650.14139626394513524No Hit
GTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTG697520.1395694078072748No Hit
CATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGGC684980.13706023190708094No Hit
TCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTAGCG683540.13677209687547973No Hit
CAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCC682600.13658400872985116No Hit
TCCCTCTAAGAAGTTTACCACGAATCGAGATTCGCGTAACTATTTAGCAG673630.13478916759550194No Hit
CTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACA667060.13347455151382145No Hit
AGCTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAG666830.13342852994627402No Hit
AAACCTTCCTAAGAGTAGAATAGGTCGGTCAACGATGCGTCCGGCTACGA666720.13340651963136002No Hit
GTGAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGC656520.13136556315751813No Hit
GTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAG652420.13054517869254248No Hit
GGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTC649010.12986285893020905No Hit
TTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACC647110.12948268076351302No Hit
CACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCAGTCCACC645150.12909049697050026No Hit
CGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTT643320.12872432536784037No Hit
TGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTC642870.128634283170465No Hit
ATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGA631840.12642724886590853No Hit
GCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCTA624280.12491453995000218No Hit
AGGTAATTATCGTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAG619320.12392207484115356No Hit
TACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCATTA612860.12262946907438703No Hit
TCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCC611420.1223413340427858No Hit
CCAGTTCTGCTTACCAAAAATGGCCCACTAGGCACTCTCATTCGTCGTCC607260.12150894395149343No Hit
GCAACCAACACCTTTTGTGGGGTCTGATGAGCGTCGACTTTGGCACTTTA602110.12047846102597522No Hit
TCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGT598490.11975412157153328No Hit
TGCAACCATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAA588630.11778119698015278No Hit
TTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTG580680.11619045149318775No Hit
TCTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAG572590.11457169287815042No Hit
AAAATAGAACCAAAGTCCTATTCCATTATTCCATGCTTATGTATTCAAGC568690.11379132716756381No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG565520.11315702991049725No Hit
CCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCT557720.11159629848932404No Hit
AGCACGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACAGATGGTAG555240.11110006593489972No Hit
CACCTCTAGCTTAAAACTCCTAAAGACTAAAGGATCGATAGGCCATGCTT553800.11081193090329852No Hit
ATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAGAT551520.11035571710326328No Hit
CACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCA544010.1088530128759542No Hit
CTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCTTTCTA542620.10857488253295024No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT541150.10828074468819068No Hit
ACTCTTCGAAAGCACATCGTGAGCGGCCGGCCGAGCCACAGACGGGGTTG536920.10743434803286213No Hit
CAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTATGCAA535630.1071762270670527No Hit
TTTTTAACTCTCTTTTCAAAGTTCTTTTCATCTTTCCCTCACGGTACTTG534430.10693611454071837No Hit
GGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGT533180.10668599732578676No Hit
CCTGACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCAT531890.10642787635997733No Hit
ACTAGGCACTCTCATTCGTCGTCCGGCTTCAATTAAGCAAGTCGGACATC530130.10607571132135366No Hit
TCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCA526610.10537138124410624No Hit
ATTCATACTGAAAATCAAAATCAAGTGAGCTTTTACCCTTTTGTTCTACA525680.10518529403619713No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA523990.10484713556160959No Hit
GATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTC522670.10458301178264182No Hit
TGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGGTTATGA519710.10399073421768378No Hit
GCACGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACAGATGGTAGC518230.10369459543520475No Hit
GAAGGACGGGACCCTAGCCACAAGAGGCATGTGCTGCATCCGCCTCGGTC511370.10232195215966011No Hit
TGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTA510760.10219989495877348No Hit
ATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC507510.1015495901999513No Hit
ACCCGATTCCCTTTCGATAGACGGCCCAAGATTGGGCACTTTGACACGGA506910.10142953393678412No Hit
TCAATTAAGCAAGTCGGACATCTTACCAATTTAAAGTTTGAGAATAGGTT505820.10121143172536377No Hit
CCGCTTCAAGTCTCAGCCCGAGAGACCCAACGCTTAGAGCCAATCCTTTT505330.10111338577711058No Hit
TCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAGCACACACA505220.1010913754621966No Hit
TCGTGAGCGGCCGGCCGAGCCACAGACGGGGTTGTCACCCTCTATGACGT501610.10036903694547414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)