FastQCFastQC Report
Fri 19 May 2023
RNA-POR-76-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-76-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49828147
Sequences flagged as poor quality0
Sequence length15-130
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCG3246740.6515875454890988No Hit
AAGAAATGTGTACAGAAGGCTGAAAGGAGAGAACTTTAGTCTTCTTGTTT2711190.5441081322971935No Hit
TTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGC2212420.44401008931758995No Hit
ACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAA2205300.4425811780638762No Hit
TTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGACATGG2137540.4289824383796572No Hit
GGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCAC1411030.2831793042594981No Hit
CCTTTTAAACCGATGCAATCTATTGGTTTAAAGACTAGCTACCAGGTGCA1359800.27289796668537564No Hit
CTATTGGAATCCTGGAGTGACCCCTGGCCTTCTCCCCGCTCCAACACCCT1319100.26472989252439993No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG1201700.24116891202075005No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA1174420.23569409474528524No Hit
TGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAG1156820.232161954567566No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGC1031890.2070897799992442No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC1008950.2024859563812397No Hit
GATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAATGGAC947390.1901314933505354No Hit
TGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAA931780.18699872584063781No Hit
ATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGTTGGAT911900.18300901295807767No Hit
TTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGATGGCCCG861110.17281597888839814No Hit
GCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG858480.17228816475956854No Hit
TTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAG855950.17178041960902138No Hit
TAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGA814140.16338957978910995No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG793540.15925537026291586No Hit
CCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGA769450.15442075339466266No Hit
GCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCG763380.15320256641291516No Hit
AAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGAA756440.15180977932010997No Hit
ATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGC739470.14840407370557046No Hit
ATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCA738020.1481130735204743No Hit
TTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTG724080.14531545794789438No Hit
CCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTG715800.14365374654610374No Hit
GGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG715210.14353533957423703No Hit
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAA713410.1431740979651521No Hit
TCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCA698250.14013164085752577No Hit
CAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGACA698000.14008146841181954No Hit
GAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCGCAGAT693870.13925261960875246No Hit
CTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGCCGAGG688640.13820301204457794No Hit
AAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAG673710.13520671358700134No Hit
GTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCT673660.1351966790978601No Hit
GATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGG669240.1343096302577738No Hit
ATTTCCACTGGGCGCCTCGGAGCTGCGGACTTCCCAGTGTGCATCGGGGC664550.13336839517632473No Hit
GTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGACAGCAG655320.1315160284808504No Hit
GGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAACGGCC637780.12799592969010065No Hit
TGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTAT629080.12624992857952355No Hit
AACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGATCCT610310.12248298135589909No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA600150.12044397316239755No Hit
CCGAGGTGAGCAGCCTCTGGTTGATAGAACAATGTAGGTAAGGGAAGTCG587400.11788517843137937No Hit
TTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATT567790.11394965179018196No Hit
AGGTCCGGACGCTCGCGTTCGGACTGTTACAGCCCGGTTCTCTCGCGGCT564930.11337567901130259No Hit
AAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTG563310.11305056156312616No Hit
AGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCAT557000.11178420903350068No Hit
AAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGC547840.10994589062282409No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC547680.10991378025757209No Hit
TCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAAC524400.1052417221134071No Hit
ATTGGATGAAGTGGAAGCGGACCGCAAGGTCCACGTTCTGGACTTAACTC516880.10373253494656344No Hit
ACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTGTAGAATAAGTGGGA507510.1018520716814936No Hit
GGTGGTAAACTCCATCTAAAGCTAAATACTGACGTGAGACCGATAGCGAA499810.10030676035374143No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAA499730.10029070517111544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)