FastQCFastQC Report
Fri 19 May 2023
RNA-POR-76-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-76-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49828147
Sequences flagged as poor quality0
Sequence length15-130
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGG3192340.6406700213034211No Hit
GTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCA3061960.6145040874187033No Hit
GGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAA3049130.6119292375050591No Hit
ACAAAGTAAACATTGAAGGGAGATGGAAGAAGGAACTCTAGCCAGAGTCT2797300.5613895294962504No Hit
GCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCT2613090.5244204646020652No Hit
AGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCCCTGTA1847510.3707763806669351No Hit
TGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCT1636500.32842882959304104No Hit
ATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCAT1602020.3215090458812366No Hit
ACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCA1551480.31136618425726326No Hit
CCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGA1527380.3065295604911818No Hit
TGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCA1476670.2963525816041283No Hit
TTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAA1396180.28019906098454755No Hit
TGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGG1377910.2765324586523356No Hit
CCGGTGCCTAATGAGTGAGCTAACTTACATTGACAGCTAGCTCAGTCCTA1370430.2750312990768049No Hit
ACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCCAT1337360.2683944879587836No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC1333410.26760176331662505No Hit
TCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTAGCG1248890.25063946287225974No Hit
CGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCCTC1178990.23661124705279526No Hit
TCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACAGA1174770.23576433616927395No Hit
TTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTA1027500.20620875185264265No Hit
AGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATT1020930.2048902199794827No Hit
TTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGT1015170.20373424683041091No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC986650.19801057422424317No Hit
GCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCTC965990.19386432331107958No Hit
GCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGT957860.1922327153767127No Hit
GAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTATGCAAAGTCCGT931570.1869565809862446No Hit
CGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATC913340.18329800624534565No Hit
ATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGA878460.17629794662041115No Hit
GCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCC856530.17189681968305986No Hit
TCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCCACGGG833480.16727092018894463No Hit
TAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAG830060.16658456113168327No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC805610.16167769594161308No Hit
TATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCT779600.15645775469033596No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC776830.15590184399191084No Hit
TTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAGCACA770640.15465957423622434No Hit
AGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGC767120.1539531462006805No Hit
CATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCA752120.15094279945830616No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT750610.15063975788624048No Hit
TACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCATTA745500.14961423309600494No Hit
ATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGT740170.14854455655354792No Hit
AAGATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGT727430.14598776872035799No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG714710.14343499468282456No Hit
AGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGG709800.14244960784915403No Hit
CATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGGC703840.14125349674351728No Hit
CACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCAGTCCACC699280.14033835133383546No Hit
AAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTAGTTGGCAT674970.13545958271336078No Hit
TGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTA674810.13542747234810878No Hit
ACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCG669150.13429156817731952No Hit
GACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCACTTCAT662790.13301518115855282No Hit
TGTAAAAGCCCTGTATTGTTATTTCTTGTCACTACCTCCCTGAGTCAGGA655640.13158024921135436No Hit
GTGAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGC654890.13142973187423565No Hit
CCCCCACCGCCCCAAGGATCTCCCGGTCCCCGCCCGGCGTGCTGACGTCA654370.1313253731871667No Hit
TCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGG643470.12913785455437465No Hit
ATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCAC627360.12590474215306463No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG626910.12581443175079338No Hit
TTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGGTTATG623650.125160183058784No Hit
ATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTT623370.12510398991959304No Hit
GGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGT614650.12335397501335942No Hit
GGAGTTTACCACCATTTTTGGGCTGCATTCCCGAACAACCCGACTCTTCG608940.12220803635342892No Hit
AGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCC608100.12203945693585595No Hit
TGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGG600460.12050618699507329No Hit
ATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAAATTTGAA589820.11837084770581574No Hit
GCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGAAT573830.1151618180784447No Hit
TTATCATTTGACAGATGTGCCGCCCCAGCCAAACTCCCAACCTGACGGTG572780.1149510938064785No Hit
AGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAGATCAA553490.11107978789578508No Hit
AAAATAGAACCAAAGTCCTATTCCATTATTCCATGCTTATGTATTCAAGC546790.10973516635085787No Hit
ACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGG534990.1073670269135234No Hit
CTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACAGAT525840.10553071540067505No Hit
TATTGTTATTTCTTGTCACTACCTCCCTGAGTCAGGATTGGGTAATTTGC515240.10340340370273052No Hit
CTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACA511970.10274714811289291No Hit
TCAATTAAGCAAGTCGGACATCTTACCAATTTAAAGTTTGAGAATAGGTT511170.10258659628663294No Hit
ACTAGGCACTCTCATTCGTCGTCCGGCTTCAATTAAGCAAGTCGGACATC511150.10258258249097643No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA505110.1013704162027137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)