FastQCFastQC Report
Fri 19 May 2023
RNA-POR-73-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-73-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51385213
Sequences flagged as poor quality0
Sequence length15-130
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGACATGG2472090.4810897640922496No Hit
TTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGC2306790.44892097654630725No Hit
ACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAA2121740.4129086708271502No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG2018370.3927919886213179No Hit
AGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCG1965440.38249135991710304No Hit
GGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCAC1800890.35046852875748513No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA1621260.3155110011901673No Hit
TGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAG1324890.2578348755701373No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC1279200.24894321251524246No Hit
GCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTTGCGAG1265850.24634518883866452No Hit
TTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAG1263520.2458917509984828No Hit
TTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGATGGCCCG1239930.24130093612728626No Hit
GCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG1168450.22739031946797614No Hit
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAA1160760.2258937799868612No Hit
CCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTG1125160.21896571684932006No Hit
TGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAA1114780.21694568046258755No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG1065670.20738845628605257No Hit
CGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGATT1044000.20317128976384702No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGC1041830.2027489892860812No Hit
TAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGA1022330.19895412324164152No Hit
TTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTG998610.19433800926348208No Hit
ATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCA994160.19347200137128942No Hit
CCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGA990130.19268772905543857No Hit
ATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGTTGGAT964370.18767461370647623No Hit
TGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTAT953460.185551434806741No Hit
GGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG953450.18554948872159No Hit
CTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGCCGAGG935540.1820640502161585No Hit
GTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCT880320.17131776801236576No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA855300.166448662964577No Hit
GGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAACGGCC827470.16103270798935873No Hit
GAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCGCAGAT803820.15643021660725626No Hit
GATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAATGGAC796590.1550231970430871No Hit
CAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGACA795730.15485583372010153No Hit
AACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGATCCT794130.1545444600959424No Hit
CTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTTGCGAGA785440.15285331209972797No Hit
ACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTGTAGAATAAGTGGGA769070.14966757070754966No Hit
AAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAG748040.1455749536350078No Hit
TTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATT737850.1435918928661442No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC731990.1424514869676613No Hit
GCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCG722930.1406883338208601No Hit
TGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAAT722850.14067276513965213No Hit
AAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGAA711570.13847758108933012No Hit
TCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCA691720.13461460206460563No Hit
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC688770.1340405069450622No Hit
ATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGC684670.13324261203315435No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT676590.13167017523115065No Hit
AGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCAT669230.13023785656001854No Hit
TTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATG669130.1302183957085086No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAA662620.12895149427521105No Hit
AAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGC659550.12835404613385568No Hit
TGATTAGAGGCATTAGGGTTAAAACAACCTTAACCTATTCTCAAACTTTA659020.12825090362085295No Hit
GATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGG653240.12712606640357801No Hit
ATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTA631190.12283494864563468No Hit
CGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGC608930.11850296309952049No Hit
GTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTT607450.11821494249717326No Hit
GTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTAC600120.11678846208149414No Hit
GTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGACGTGCA596580.11609954793804203No Hit
GGATTGTCCGGAGATAGGGTTTAATGCCTGGTAAAGCACCACACTTCTTG596390.11606257232017311No Hit
AAGTCTTGTAATTGGAATGAGTACAACTTAAATCCTTTAACGAGGATCCA591700.11514985838435661No Hit
AAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTG585090.1138634960995491No Hit
GTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGACAGCAG584970.11384014307773717No Hit
ACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCGAAAAGTCC578080.11249929040870181No Hit
AATGCGCCCTTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGC571850.11128687935963212No Hit
AAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAG555130.10803302498716898No Hit
CCACACTTCTTGTGGTGTCCGGTGCGTTCTCGACGGCCCTTGAAAATCCG555130.10803302498716898No Hit
TAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGATTCGT553940.1078014408542006No Hit
AGGGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAACGG551220.10727210569313003No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAACTCAGTGTACA538310.10475970976319589No Hit
GCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGA535840.1042790267309002No Hit
CTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGACAT535510.10421480592091736No Hit
TTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAACCTTAA528700.10288952193308998No Hit
GTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATGGCGCT527770.10270853601404746No Hit
CTTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGA525680.10230180421748956No Hit
AATCTCCGACGCTTGCGTCGGCGAGTTGTAGTTGCGAGAAGCACTTTCTA519080.10101738801783307No Hit
TGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGC518470.10089867682362239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)