FastQCFastQC Report
Fri 19 May 2023
RNA-POR-73-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-73-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51385213
Sequences flagged as poor quality0
Sequence length15-130
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGG3928230.7644670072691924No Hit
GGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAA3364130.6546883439015811No Hit
GCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCT2767120.5385051143020464No Hit
TGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCA2689620.5234229543818374No Hit
GTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCA2498710.48627024276419756No Hit
TGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCT2275180.442769401384013No Hit
AGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCCCTGTA2073150.40345264307846695No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC1894810.3687461604956274No Hit
ATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCAT1619790.3152249266729711No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC1487060.2893945384638184No Hit
TCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACAGA1380250.26860840296604394No Hit
TTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAA1365630.26576322647528966No Hit
CCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGA1347160.26216880720140245No Hit
TTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTA1330780.25898111972407317No Hit
AAGATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGT1296400.2522904789749533No Hit
TGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGG1257720.24476302161090582No Hit
CATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCA1238810.24108297459037487No Hit
ATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGT1221890.2377901985148918No Hit
TCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCCACGGG1213440.23614575656230127No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC1180770.2297878963740016No Hit
CGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCCTC1118460.21766183979815362No Hit
ACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCCAT1111460.21629958019245732No Hit
TTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGGTTATG1097770.21363538962074555No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG1068060.2078535706371403No Hit
ACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCA1047260.2038057135230713No Hit
CGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATC1047170.20378819875671236No Hit
AGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATT1046930.2037414927130885No Hit
AGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGG1021920.19887433375045072No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG1018300.19816985092579067No Hit
GCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCC998980.19441001441406888No Hit
TATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCT985520.19179058380083003No Hit
TCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTAGCG969620.18869630841074844No Hit
TAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAG962950.18739826961503497No Hit
TTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGT934940.18194728510709882No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA876680.17060939301740366No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC874330.1701520630069199No Hit
AGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCC869080.1691303683026477No Hit
GCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCTC844680.1643819205342206No Hit
GAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTATGCAAAGTCCGT805060.1566715311659796No Hit
ACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCG804720.1566053642708458No Hit
CTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGG799430.15557588522596957No Hit
TTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAGCACA769980.1498446644562902No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT767350.14933284406157857No Hit
ATTAACCCGATTCCCTTTCGATAGACGGCCCAAGATTGGGCACTTTGACA766960.14925694674068976No Hit
GTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAG766510.149169372908895No Hit
TGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTA756740.1472680477163732No Hit
GCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGAAT755950.1471143069894446No Hit
AGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCT755760.1470773313715757No Hit
TTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACA752710.14648377540052235No Hit
TTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACC752060.14635727986570768No Hit
AAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTAGTTGGCAT743270.14464667101798334No Hit
CAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATTCCC741650.14433140522352217No Hit
TGCAACCATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAA706960.13758043583472157No Hit
TCCCTCTAAGAAGTTTACCACGAATCGAGATTCGCGTAACTATTTAGCAG706690.1375278915356447No Hit
GGAGTTTACCACCATTTTTGGGCTGCATTCCCGAACAACCCGACTCTTCG701310.13648089772440955No Hit
AGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGC692240.13471579849245735No Hit
GACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCACTTCAT682090.13274052206419773No Hit
TCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGG680890.13250699184607836No Hit
ATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTT676080.1315709248884499No Hit
ACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCT672130.130802221253807No Hit
GGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGT668540.1301035766845999No Hit
GCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCG667070.12981750216740368No Hit
TGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGG665350.12948277552143259No Hit
CAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCC661970.12882499874039638No Hit
CTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACA655100.12748803824166302No Hit
TCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCC649140.12632817149167017No Hit
GGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGT634160.12341293593548011No Hit
ATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAGAT631830.12295949809529834No Hit
AAAATAGAACCAAAGTCCTATTCCATTATTCCATGCTTATGTATTCAAGC622930.12122748231091307No Hit
GACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCCA619840.1206261419992557No Hit
TCTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAG618080.12028363101268062No Hit
ATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAAATTTGAA613340.11936118665110915No Hit
GCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCTA608050.11833170760623295No Hit
CCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTC602560.11726330685833686No Hit
CACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCAGTCCACC602240.11720103213350502No Hit
ATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCAC600950.1169499871490267No Hit
TGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTC588160.11446094424090447No Hit
TGACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCC585080.11386155001439811No Hit
CTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACAGAT584810.11380900571532125No Hit
TTGGCAAATGCTTTCGCAGTAGTTCGTCTTTCGTAAATCCAAGAATTTCA580050.11288266918344778No Hit
TCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAGCACACACA566280.11020290993052806No Hit
TTATCATTTGACAGATGTGCCGCCCCAGCCAAACTCCCAACCTGACGGTG559230.10883091989907681No Hit
AGCTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAG559130.10881145904756687No Hit
CATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGGC555300.10806610843473588No Hit
ACCAAAGTCCTATTCCATTATTCCATGCTTATGTATTCAAGCGCTGGCTT551510.1073285421625089No Hit
CCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCT551070.10724291441586513No Hit
AAACCTTCCTAAGAGTAGAATAGGTCGGTCAACGATGCGTCCGGCTACGA549970.10702884504925571No Hit
CGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTT547990.10664352018935874No Hit
GTGAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGC547030.10645669601486325No Hit
TTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTG545810.1062192736264419No Hit
GTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTG543100.10569188455052234No Hit
CTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCTTTCTA539830.10505551470614707No Hit
CTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGA534890.1040941486415557No Hit
GGACACGAAAATCATTGTCTCCCGCGGATTTTCAAGGGCCGTCGAGAACG533100.10374579939952763No Hit
CCAGTTCTGCTTACCAAAAATGGCCCACTAGGCACTCTCATTCGTCGTCC532710.10366990207863885No Hit
ATTCATACTGAAAATCAAAATCAAGTGAGCTTTTACCCTTTTGTTCTACA528760.10290119844399595No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCCTTCCTGACTTTGGTTGGAT524660.10210330353208812No Hit
GGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTC520360.10126648691716039No Hit
TGTAAAAGCCCTGTATTGTTATTTCTTGTCACTACCTCCCTGAGTCAGGA518140.10083445601363956No Hit
CTTTCCGGGGCCCCCGCCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACT517190.10064957792429506No Hit
AGGTAATTATCGTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAG516370.1004899989419135No Hit
TATTGTTATTTCTTGTCACTACCTCCCTGAGTCAGGATTGGGTAATTTGC516260.10046859200525257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)