FastQCFastQC Report
Fri 19 May 2023
RNA-POR-71-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-71-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50831351
Sequences flagged as poor quality0
Sequence length15-130
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGACATGG1922970.3783039329409128No Hit
TTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGC1842900.362551843251225No Hit
AGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCG1836720.3613360581346736No Hit
ACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAA1725270.3394106129502637No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG1664770.3275085094629887No Hit
GGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCAC1339370.2634928983099426No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA1294510.2546676361208657No Hit
TGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAG1065750.20966391391013783No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC981810.19315048305523103No Hit
GCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG906520.1783387579055296No Hit
TTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGATGGCCCG895540.17617867366932663No Hit
TTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCCAG866900.17054435558873893No Hit
TGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAA835980.16446149542631672No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG834110.16409361222761912No Hit
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAA821550.16162269619786418No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGC782320.15390501818454522No Hit
GGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG770860.15165050403637706No Hit
CCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTG745780.14671654113619761No Hit
CGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGATT732240.14405283070284713No Hit
ATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGTTGGAT721400.14192028852430066No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA713790.1404231809616864No Hit
ATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCA706950.13907755471618294No Hit
GTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCT704580.13861130702585497No Hit
TAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCCAGTAACGGCGAGTGA696130.13694894711730168No Hit
TTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTG694890.13670500317805837No Hit
TGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTAT681690.13410818059901655No Hit
GGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAACGGCC650480.12796826903144873No Hit
GAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCGCAGAT629290.12379958187615356No Hit
CAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGACA623290.1226192079765891No Hit
AACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGATCCT617970.12157260978564194No Hit
AAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAG615150.12101783405284665No Hit
ATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGC614700.1209293060103793No Hit
CTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGCCGAGG608210.11965253490901707No Hit
TCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCA607020.11941842741893678No Hit
TTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATT599220.11788394134950297No Hit
CCCCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGA591070.11628060013592792No Hit
GCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTTGCGAG583340.11475988509532238No Hit
AGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCAT575250.11316834762074295No Hit
GATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAATGGAC573140.11275324946606279No Hit
ACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTGTAGAATAAGTGGGA566690.111484347524031No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAA558060.10978657639849076No Hit
TGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAAT528570.10398503868213142No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC527280.10373125829372506No Hit
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC520590.10241514139571069No Hit
GCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCG519960.10229120213625642No Hit
GATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGG513660.10105180954171372No Hit
AAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTG511100.10054818334456622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)