FastQCFastQC Report
Fri 19 May 2023
RNA-POR-71-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-71-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50831351
Sequences flagged as poor quality0
Sequence length15-130
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGG2888130.5681788784248524No Hit
GGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAA2610440.5135492070631764No Hit
GCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCT2282530.44903980616214584No Hit
GTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCA2017700.3969400695252031No Hit
TGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCT1846990.36335646479276146No Hit
TGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCA1807030.35549517462166214No Hit
AGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCCCTGTA1743570.3430107533439353No Hit
ATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCAT1450160.28528850236540043No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC1269120.24967268723587538No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC1223870.24077070074332668No Hit
TTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAA1130140.22233129314229716No Hit
TCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACAGA1078460.21216433928738193No Hit
CCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGA1059030.20834189514262563No Hit
TGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGG1044150.2054145678717058No Hit
CATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCA979440.19268423536490303No Hit
TTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTA978770.19255242694611835No Hit
ATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGT972290.19127762313458874No Hit
AAGATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGT954880.18785257153601917No Hit
ACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCA933930.1837310993367066No Hit
CGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCCTC903560.1777564401150778No Hit
TCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTAGCG888560.17480550536616662No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC861930.16956661254193303No Hit
ACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCCAT858780.16894691624466168No Hit
TCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCCACGGG832870.16384966828837583No Hit
TTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGGTTATG811210.1595885185109481No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG797680.15692677536743022No Hit
AGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGG774270.1523213498692962No Hit
GCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCTC767530.1509953965221188No Hit
TTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGT744730.14650997570377383No Hit
TAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAG741940.14596110184047636No Hit
AGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATT741300.1458351952911895No Hit
CGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATC733220.14424562510644268No Hit
TATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCT732890.14418070454196663No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG732040.14401348490619498No Hit
GCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCC717520.141156980069249No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC679830.13374226469015157No Hit
TGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTA665070.130838544897223No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT654810.12882010552896775No Hit
GAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTATGCAAAGTCCGT644420.12677609139288862No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA619130.1218008154062244No Hit
TTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAGCACA600080.11805312827510722No Hit
GCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGAAT596260.11730162355905117No Hit
GTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAG588660.11580648328626954No Hit
ACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCG581080.1143152775931531No Hit
AAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTAGTTGGCAT574360.1129932588256409No Hit
AGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCC570050.11214535690778708No Hit
TTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACC567840.11171058585478083No Hit
ATTAACCCGATTCCCTTTCGATAGACGGCCCAAGATTGGGCACTTTGACA559710.11011117922087099No Hit
AGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGC550280.10825602490872217No Hit
TGCAACCATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAA542540.10673334257828401No Hit
TTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACA540750.10638119769824728No Hit
CTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGG539850.10620414161331261No Hit
GGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGT534120.10507688453922856No Hit
CACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCAGTCCACC533980.10504934248157206No Hit
AGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCT523280.10294434236068209No Hit
GACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCACTTCAT521720.10263744514679533No Hit
CATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGGC509820.10029637024599249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)