FastQCFastQC Report
Fri 19 May 2023
RNA-POR-82-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-82-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49279402
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTG2179490.44227200646631226No Hit
CGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCA1942300.39414033473863985No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC1938630.39339560167552357No Hit
GGGACTCTGTAGGGACCAGAACAAAGTAAACATTGAAGGGAGATGGAAGA1855690.3765650402981757No Hit
CTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACC1706320.34625420170480153No Hit
CCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGC1266680.25704045678151693No Hit
CCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATA1242300.25209315648757263No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCATTCCAATTACA1093400.22187769242816702No Hit
CCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGT1022490.20748831327133394No Hit
GTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCA991880.20127679309095511No Hit
CTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAAT976870.1982308957401715No Hit
CCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCA856580.1738211027804274No Hit
CTCTAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTAT804620.16327714366339105No Hit
GTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCG797980.1619297247154095No Hit
CTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTGC779330.15814518203772035No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA772860.15683226026159977No Hit
CCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGC772180.15669427157415586No Hit
CCCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAAT747990.1517855269428797No Hit
CGCCGGGAACTGCCGCTGGCCCCCCACCGCCCCAAGGATCTCCCGGTCCC688690.13975210169961072No Hit
CCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGA677240.13742861571250398No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG661690.1342731391099267No Hit
CTGCGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC661280.13418994004837964No Hit
CTCCACTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA644250.13073413512607154No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCC642800.13043989454255148No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCAACCAGAGGCTGCTCACCTC582430.11818934004109871No Hit
CAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCA571720.11601601821385739No Hit
CACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCT550610.11173228116688591No Hit
CCCAGCACGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGA550610.11173228116688591No Hit
CTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACAC545320.11065881034838856No Hit
CTTTTCACAAGGTCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCC542200.11002568578246952No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCA520970.10571759779065501No Hit
CCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATAT512520.10400288542462427No Hit
CTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCC508350.10315669009132863No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA499690.10139936357182255No Hit
TTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATT498840.10122687771251769No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)