FastQCFastQC Report
Fri 19 May 2023
RNA-POR-79-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-79-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50299323
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATG2769680.5506396179527108No Hit
CTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAA2421960.48150946286096136No Hit
GTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATG2397170.47658096710367254No Hit
CTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTT2244030.44613522929523325No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA1666830.3313821937523891No Hit
ATTAGATTAAAAACCAATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCAT1403320.2789938146881222No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1315890.2616118709987409No Hit
CGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA1307620.25996771368075866No Hit
GGCAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAA1254390.24938506627614054No Hit
GTGAAATTCTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCA1201960.2389614667378326No Hit
TCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAG1182330.2350588297182449No Hit
GGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA1178550.23430732854992897No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA1147500.2281342832387625No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG1116800.22203082136910668No Hit
CGCAGATTCAGACAATTGGCATTTGCACTTGCCTGAAAAGACAATGGTGC1115160.2217047732431707No Hit
CGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGG1084880.2156848115033278No Hit
CCGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC1082900.2152911680342099No Hit
CGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGT1080740.21486173879517226No Hit
GCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATT1064670.21166686478066515No Hit
AAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAG1056760.21009427900252256No Hit
AGAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAA1035880.2059431296918251No Hit
GTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCA1018510.2024898028945638No Hit
GTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGA982120.1952551130757764No Hit
CTCACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTT927270.18435039374188No Hit
CGAACGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAA910780.18107201959756No Hit
CAGGCCCCGACGAGTAGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCG904310.17978571997877585No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA892120.17736222811587343No Hit
ATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAG890490.1770381680882663No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGACCTTGTGAAAAGACATGA860060.17098838487349025No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC837530.16650919933852787No Hit
GTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAA837330.16646943737195033No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG808780.16079341664300334No Hit
CCGAACGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAA806300.16030036825744157No Hit
CTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTC788370.15673570795376313No Hit
ACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGAT765800.15224857002548525No Hit
GTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAACCT765260.1521412127157258No Hit
CGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACG757310.15056067454426772No Hit
CAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGA744920.14809742071478776No Hit
GGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTC720640.14327031797227172No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAA703270.13981699117501045No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT699230.1390137994501437No Hit
GGAGTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGA697530.13867582273423443No Hit
CGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTG686520.13648692647413962No Hit
AAGGAGTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCA655500.13031984545795974No Hit
GAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAAC650900.1294053202266758No Hit
CTGGAACTCAGTGTACAGTTTTATCAGGTAAAGCGAATGATTAGAGGCAT643130.12786056782513752No Hit
AAAGAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGA642940.12782279395688884No Hit
CAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTC641960.1276279603206588No Hit
CAGGCTTTCACCCTGAAATCGGATTGTCCGGAGATAGGGTTTAATGCCTG636390.1265205895514737No Hit
CGGGAATGCAGCCCAAAAATGGTGGTAAACTCCATCTAAAGCTAAATACT629050.12506132537807715No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACT620460.12335354891357088No Hit
GTTTAATGCCTGGTAAAGCACCACACTTCTTGTGGTGTCCGGTGCGTTCT617590.12278296469318285No Hit
GACTAATCGAACTGTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA615190.1223058210942521No Hit
TGAAATTCTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCAA593990.11809105263703053No Hit
GTGAACTATGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCT590170.11733159907539908No Hit
GTCAGGCCCCGACGAGTAGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGG586940.11668944331517145No Hit
CTCAGCAATGCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGG585730.11644888341737722No Hit
GTTGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAGCAACTAG583800.11606518043990374No Hit
TGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAG553570.11005515919170522No Hit
CACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGA550540.10945276539805517No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA547670.10888218117766715No Hit
CGACTCTCGTAGCCGGACGCATCGTTGACCGACCTATTCTACTCTTAGGA547340.10881657393281416No Hit
GAATGATTAGAGGCATTAGGGTTAAAACAACCTTAACCTATTCTCAAACT547280.1088046453428409No Hit
CGACGAATGAGAGTGCCTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGA542020.10775890562185102No Hit
CCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATT540640.10748454805246584No Hit
GTCCGACTTGCTTAATTGAAGCCGGACGACGAATGAGAGTGCCTAGTGGG535290.10642091544651605No Hit
CGGCCTCTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAAC526520.10467735321208996No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAAC525160.10440697183936253No Hit
CCCTGTTGAGCTTGACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTG522450.10386819719223656No Hit
AGCAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAAGGTTCCA522340.1038463281106189No Hit
CAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAAC520420.10346461323147431No Hit
CTCATAACCGCAGCAGGTCTCCGAGGTGAGCAGCCTCTGGTTGATAGAAC518690.10312067222057839No Hit
CTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAA517250.10283438606121995No Hit
GTTTATTTGATTGTACCATTACTACTTGGATAACCGTAGTAATTCTAGAG516400.10266539770326531No Hit
GACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGT514600.10230754000406725No Hit
CTGAAATCGGATTGTCCGGAGATAGGGTTTAATGCCTGGTAAAGCACCAC512770.10194371800988256No Hit
CGGACTTTGCATAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCT512120.10181449161850548No Hit
CTCAGGATAGCTGGAACTCAGTGTACAGTTTTATCAGGTAAAGCGAATGA511320.10165544375219523No Hit
GGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGC510450.10148247919758284No Hit
AAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTGAAGCGGG510230.10143874103434752No Hit
GTTAAAACAACCTTAACCTATTCTCAAACTTTAAATTGGTAAGATGTCCG504920.10038306082171325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)