FastQCFastQC Report
Fri 19 May 2023
RNA-POR-76-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-76-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50199897
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCA2984330.5944892675775809No Hit
CTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACC2927880.5832442245847635No Hit
GGGACTCTGTAGGGACCAGAACAAAGTAAACATTGAAGGGAGATGGAAGA2876100.572929462385152No Hit
CCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTG2844290.566592795997171No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC2630900.5240847406519579No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCATTCCAATTACA1805180.35959834738306334No Hit
CCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGT1639380.3265703911703245No Hit
CCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATA1518780.3025464374956785No Hit
CAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCA1474790.293783471308716No Hit
CCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGC1443060.28746274120841325No Hit
CTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAAT1425900.28404440750147353No Hit
CAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACAT1371030.2731141061902976No Hit
CTCTAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTAT1369100.2727296432500648No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG1342370.2674049311296396No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCC1338490.2666320211772546No Hit
CTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTGC1331600.26525950840098333No Hit
CTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCC1259930.25098258667741885No Hit
CCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGC1181160.23529131942242829No Hit
CTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACAC1062130.21158011539346386No Hit
CCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCA1005540.20030718389721No Hit
CTGCGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC1004910.2001816856317454No Hit
CTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGTT971300.19348645277100868No Hit
GTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCA963970.1920262904125082No Hit
TGCCGCTCACTTTGATGTCTGCAGGCCAGATGAGGGCTCCAGATGGCACA955410.1903211076309579No Hit
CTCCACTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA953970.19003425445275315No Hit
CTTTTCACAAGGTCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCC942400.18772946884731656No Hit
GTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCG911140.18150236443712225No Hit
CTAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTATCC881320.17556211320513265No Hit
CACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCT856490.17061588791706087No Hit
CTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCC834480.16623141676963998No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA829380.1652154784301649No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCA786520.15667761230665475No Hit
CTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAG770980.15358198842519538No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA758480.15109194347550156No Hit
CTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAA750640.1495301872830536No Hit
CCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGA748080.1490202260773563No Hit
CAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGA733480.14611185357611392No Hit
CCCAGCACGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGA719070.14324132975810688No Hit
CCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGC710410.141516226616959No Hit
AAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGG710190.1414724018258444No Hit
CTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAG703590.14015765809240605No Hit
CCCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAAT690610.13757199541664397No Hit
CAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACC679890.13543653286778656No Hit
CTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTT672140.1338927049989764No Hit
CGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCC664880.13244648689219424No Hit
CGCCGGGAACTGCCGCTGGCCCCCCACCGCCCCAAGGATCTCCCGGTCCC662250.13192258143477864No Hit
CTCATTCCAATTACAAGACTTGTAAAAGCCCTGTATTGTTATTTCTTGTC659210.1313170025030131No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA659040.13128313789169727No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCATCACTTGGCGAATTCTGC632820.1260600196052195No Hit
CTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC622410.1239863101711145No Hit
CCTATACCCAAATTTGACGATCGATTTGCACGTCAGAATCGCTACGAGCC621570.12381897915049508No Hit
CCCAGTCCCAACCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGG617800.1230679815936674No Hit
ACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATC617080.12292455500456505No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA603720.12026319496233229No Hit
CTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAGT595920.11870940691372335No Hit
CCTCACGGTACTTGTTCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGA585770.11668749041457196No Hit
CCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGAC573850.11431298355054395No Hit
CTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAGCC566680.11288469376739957No Hit
CCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATAT562080.11196835722591222No Hit
CAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGG561630.11187871560772325No Hit
CTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCATTATT554510.11046038600437766No Hit
CCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAG549730.10950819281561473No Hit
CATGCTTTCACAGTTTGTATTCATACTGAAAATCAAAATCAAGTGAGCTT545990.10876317136666634No Hit
GTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATTC545590.10868348992827614No Hit
CAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTG538890.10734882583524026No Hit
CAAGACTTGTAAAAGCCCTGTATTGTTATTTCTTGTCACTACCTCCCTGA521130.10381096997071529No Hit
CTAGCCACAAGAGGCATGTGCTGCATCCGCCTCGGTCGGGCCCGAGCCGC509890.10157192155195059No Hit
CTCTCATTCGTCGTCCGGCTTCAATTAAGCAAGTCGGACATCTTACCAAT508200.10123526747475198No Hit
CTTCAAACGGTTTCACGTACTTTTTAACTCTCTTTTCAAAGTTCTTTTCA503240.10024721763871348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)