FastQCFastQC Report
Fri 19 May 2023
RNA-POR-73-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-73-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51750431
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATG2491460.4814375362400363No Hit
GTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATG2318450.44800593061727356No Hit
CTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTT2160910.4175636720784026No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA1995540.38560838266255215No Hit
CTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAA1977940.38220744480369645No Hit
ATTAGATTAAAAACCAATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCAT1804540.34870047748974303No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1604420.3100302681537087No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG1323330.25571381231588197No Hit
CTCACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTT1272430.24587814543998676No Hit
GGCAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAA1270690.24554191635621356No Hit
TCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAG1236690.23897192276524226No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC1196890.2312811655616936No Hit
GTGAAATTCTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCA1165880.22528894493651658No Hit
GGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA1127730.2179170256572356No Hit
CGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA1117040.21585134237819195No Hit
CGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGT1078790.20846009958834932No Hit
AGAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAA1067850.2063461075329015No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA1051350.2031577282902243No Hit
GTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAA1047000.20231715558079122No Hit
GCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATT1025610.1981838566716478No Hit
AAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAG1000170.19326795558475637No Hit
CGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGG998090.19286602656507346No Hit
CCGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC979780.1893278917812298No Hit
CGCAGATTCAGACAATTGGCATTTGCACTTGCCTGAAAAGACAATGGTGC963410.18616463310228276No Hit
GTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGA955890.18471150510804443No Hit
ACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGAT947080.1830091038275604No Hit
ATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAG933120.18031154175314984No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA889630.17190774700987516No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT841900.16268463541878522No Hit
CAGGCCCCGACGAGTAGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCG829100.16021122606689014No Hit
GTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCA804850.1555252747556827No Hit
CGAACGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAA798440.154286637728679No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG797990.15419968193115147No Hit
CTCAGCAATGCGCCCTTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGG793730.15337650038122388No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGACCTTGTGAAAAGACATGA780990.1509146851356658No Hit
GACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGT768510.14850311101756813No Hit
GGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTC766070.14803161735986314No Hit
CCGAACGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAA734310.14189447040547354No Hit
CAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGA725780.1402461749545622No Hit
CGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACG721000.13932251114971392No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAAC718050.13875246758814436No Hit
CTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTC717680.13868097059906612No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACT696260.13454187463675424No Hit
GGAGTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGA692600.13383463415019672No Hit
CAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTC689170.13317183773793112No Hit
AAGGAGTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCA677610.1309380399170009No Hit
AAAGAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGA670250.1295158295396612No Hit
CACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGA665820.1286597980217788No Hit
GTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAACCT665370.12857284222425122No Hit
GAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAAC660750.12768009603630162No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA660600.12765111077045907No Hit
CCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATT655460.12665788232758873No Hit
GACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTG647630.12514485145060916No Hit
GGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC609550.11778645862872136No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAA606320.11716230923757909No Hit
CAGGCTTTCACCCTGAAATCGGATTGTCCGGAGATAGGGTTTAATGCCTG605450.11699419469569249No Hit
CTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACC603530.11662318329290823No Hit
TAACAATACAGGGCTTTTACAAGTCTTGTAATTGGAATGAGTACAACTTA596540.11527246990464678No Hit
GTGAACTATGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCT591620.11432175318501211No Hit
AGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAA587260.11347924812452287No Hit
GTTTATTTGATTGTACCATTACTACTTGGATAACCGTAGTAATTCTAGAG578180.1117246733655223No Hit
GGGAAACGAATGGACTCAGCAATGCGCCCTTTGAGATTCAGCCGGGCGGT571750.11048217163640627No Hit
CGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTG566630.10949280789564825No Hit
TGAAATTCTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCAA556360.1075082833609637No Hit
GTTTAATGCCTGGTAAAGCACCACACTTCTTGTGGTGTCCGGTGCGTTCT555610.1073633570317511No Hit
ATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAG555520.10734596587224558No Hit
GTCAGGCCCCGACGAGTAGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGG551550.10657882250294688No Hit
GACTAATCGAACTGTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA542060.10474502135064344No Hit
AGCAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAAGGTTCCA540620.10446676279855524No Hit
TGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAG536000.10357401661060563No Hit
GTTGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAGCAACTAG528820.10218658855227697No Hit
CTGGAACTCAGTGTACAGTTTTATCAGGTAAAGCGAATGATTAGAGGCAT525250.10149673922522501No Hit
AATGGACTCAGCAATGCGCCCTTTGAGATTCAGCCGGGCGGTTGTGGCGG521950.10085906337668955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)