FastQCFastQC Report
Fri 19 May 2023
RNA-POR-73-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-73-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51750431
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTG3489770.6743460745283455No Hit
CGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCA3255990.6291715715372497No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC2783270.5378254724100753No Hit
CCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGC2645820.5112653071430457No Hit
CTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACC2373580.4586589819899278No Hit
CCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGT2275780.43976058866060463No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCATTCCAATTACA2022410.39080060995047555No Hit
GTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCA1866960.3607622127823438No Hit
CCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATA1539050.2973984892995384No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCC1495370.2889579798861965No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG1314690.2540442610033528No Hit
CCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCA1306180.2523998302545538No Hit
CCCAGCACGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGA1280940.24752257618878576No Hit
CTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAAT1249160.2413815645322838No Hit
CTCTAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTAT1245440.2406627299393893No Hit
CTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACAC1234630.23857385844767168No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA1205550.23295458157633506No Hit
CCCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAAT1150020.2222242361614341No Hit
CCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGC1123170.21703587357562296No Hit
CCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGA1107070.2139247883751925No Hit
CTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTGC1098200.21221079298837145No Hit
GTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCG1042530.2014533946586841No Hit
CTGCGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC1020350.19716744001610345No Hit
ACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATC1012170.19558677685215803No Hit
CACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCT996070.1924756916517275No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCA991950.19167956301658629No Hit
CTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCC979830.18933755353651066No Hit
CAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCA973200.18805640478627125No Hit
CTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCC968170.18708443220501872No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA940610.1817588726942197No Hit
CTCCACTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA869450.16800826257852808No Hit
CTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGTT848600.1639793106264178No Hit
GTCGGATTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTC822110.15886051267862872No Hit
CCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGAC821940.15882766271067386No Hit
CTTTTCACAAGGTCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCC813660.15722767603616675No Hit
CCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGC782800.15126444067683223No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCAACCAGAGGCTGCTCACCTC775050.14976686860830202No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA774670.14969343926816764No Hit
CTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAG771260.14903450755801434No Hit
CCGTGTTCCGGTTCGGGAATATTAACCCGATTCCCTTTCGATAGACGGCC765220.14786736752008886No Hit
CAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACC748200.1445785060224909No Hit
CCCAGTCCCAACCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGG740190.14303069282650033No Hit
CCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATAT738070.14262103440259272No Hit
TTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATT737160.1424451904564814No Hit
CGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCC736670.1423505052547292No Hit
GTTCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCAC726730.14042974830489818No Hit
CCTAATTCTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCAT713640.13790030077237425No Hit
CTTTGGTTGGATACCAACAGTCCCTCTAAGAAGTTTACCACGAATCGAGA713000.1377766303047795No Hit
GCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAG708690.13694378699957108No Hit
CTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTT693230.13395637226673532No Hit
CCCTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGAC686860.1327254646439563No Hit
TACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCAT681200.1316317539461652No Hit
CCTATACCCAAATTTGACGATCGATTTGCACGTCAGAATCGCTACGAGCC663870.12828298956582604No Hit
GTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTG657590.1270694731025525No Hit
CTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAA657470.1270462848898785No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA654880.12654580596633097No Hit
GCTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAAC650090.12562020981042651No Hit
CTAGCCACAAGAGGCATGTGCTGCATCCGCCTCGGTCGGGCCCGAGCCGC649820.12556803633190997No Hit
GTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACT649460.12549847169388792No Hit
GTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATTC640610.12378834100917922No Hit
CCTCACGGTACTTGTTCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGA637280.12314486810747528No Hit
CTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCATTATT630640.12186178700617971No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGC619490.11970721557855238No Hit
CTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAGT619370.11968402736587835No Hit
CCTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTG608330.11755071179986888No Hit
CTCCGGAATCGAACCCTAATTCTCCGTCACCCGTTGCAACCATGGTAAGC604830.11687438893021009No Hit
CTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAG604580.11682608015380587No Hit
CCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAG596610.11528599636203996No Hit
CTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC593030.11459421468393181No Hit
CGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGT589990.11400677996285674No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTT584060.1128608957865491No Hit
TCCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATG581040.11227732576758635No Hit
CCCCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAA574580.11102902698530183No Hit
CTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACA562820.10875658214324824No Hit
AAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGG560930.10839136779363248No Hit
GTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTAT560730.10835272077250911No Hit
CGCCCCTATACCCAAATTTGACGATCGATTTGCACGTCAGAATCGCTACG559980.10820779444329653No Hit
TGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATT557460.10772084197714218No Hit
CCCTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGT551070.10648606965225083No Hit
CAACAGACACGCTCTTACTCAAACCTTCCTAAGAGTAGAATAGGTCGGTC549840.10624839047234215No Hit
CCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCATTATTCCATGCTT549470.10617689348326392No Hit
CTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAGCC548910.10606868182411852No Hit
CATGCTTTCACAGTTTGTATTCATACTGAAAATCAAAATCAAGTGAGCTT544200.1051585444766634No Hit
CGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAG543210.10496724172210277No Hit
TCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCG538610.10407836023626547No Hit
TCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTT533020.10299817599586755No Hit
GCTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATC531310.10266774396526282No Hit
ATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCC528910.10220397971178251No Hit
GTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTA528130.10205325632940139No Hit
CAAGACTTGTAAAAGCCCTGTATTGTTATTTCTTGTCACTACCTCCCTGA522250.10091703390837461No Hit
CTCATTCCAATTACAAGACTTGTAAAAGCCCTGTATTGTTATTTCTTGTC521450.10076244582388116No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCATCACTTGGCGAATTCTGC520780.1006329783031179No Hit
GTTAAAAAGAAAAGAGAACTCTTTCCGGGGCCCCCGCCGGCGTCTCCGCG520380.10055568426087119No Hit
CCCCAGCCAAACTCCCAACCTGACGGTGTCTTGGGCTTGGATCCTCCCGT519780.10043974319750111No Hit
CCATGCTTTCACAGTTTGTATTCATACTGAAAATCAAAATCAAGTGAGCT519590.10040302852743391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)