FastQCFastQC Report
Fri 19 May 2023
RNA-POR-71-S1-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-71-S1-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51248209
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATG1939070.37836834454058677No Hit
GTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATG1851770.36133360289722516No Hit
CTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAA1845940.3601960021666318No Hit
CTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTT1752560.3419748776001128No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA1649790.3219214938808886No Hit
ATTAGATTAAAAACCAATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCAT1339660.26140620836134976No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1280090.2497823875171911No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG1066150.20803653840859102No Hit
GGCAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAA1009140.19691224721628806No Hit
CTCACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTT976690.19058031862147612No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC921720.17985409012049572No Hit
TCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAG893370.17432218948373396No Hit
CGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA840170.16394133890610693No Hit
AGAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAA835320.16299496437036462No Hit
GTGAAATTCTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCA824790.1609402584195674No Hit
GCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATT809510.15795869081005348No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA791980.15453808346746323No Hit
ACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGAT764670.14920911675176787No Hit
GGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA748410.1460363229474029No Hit
GTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAA734890.1433981819735398No Hit
CGCAGATTCAGACAATTGGCATTTGCACTTGCCTGAAAAGACAATGGTGC721170.14072101524562544No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA712240.13897851532723807No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT703800.1373316285062762No Hit
CCGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC697880.13617646618635978No Hit
CGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGG695710.13575303675490397No Hit
GTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGA684390.13354417907560437No Hit
CAGGCCCCGACGAGTAGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCG651660.1271576144251207No Hit
GTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCA630450.12301893320798782No Hit
CGAACGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAA625550.1220628022337327No Hit
GACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGT623340.1216315676514666No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG620990.12117301504136467No Hit
CAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTC618960.12077690363774468No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACT610390.11910465007664951No Hit
ATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAG607810.11860121784938865No Hit
AAAGAAACTAACAAGGATTCCCCCAGTAACGGCGAGTGAAGCGGGAATAG599610.11700116193328824No Hit
CGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACG594200.11594551528620249No Hit
CTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTC590610.11524500300098292No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGACCTTGTGAAAAGACATGA579390.11305565819870896No Hit
CTCAGCAATGCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGG573410.11188878815257719No Hit
CACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGA572890.11178732119204401No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA557120.10871014048510455No Hit
CCGAACGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAA528240.10307482160010704No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAAC524990.10244065309677455No Hit
GGAGTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGA524230.10229235523137989No Hit
CGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGT516340.10075278923405888No Hit
CCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATT514930.10047765766799772No Hit
AGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAA512690.10004056922262397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)