FastQCFastQC Report
Wed 26 Apr 2023
gonad_1_val_1.fastp-trim.20230426.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamegonad_1_val_1.fastp-trim.20230426.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64068570
Sequences flagged as poor quality0
Sequence length15-131
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1922820.300119075546715No Hit
AACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1497270.23369805194653165Illumina PCR Primer Index 6 (100% over 42bp)
AACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA645810.10079981494826558Illumina PCR Primer Index 6 (100% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)