FastQCFastQC Report
Wed 15 Feb 2023
E1_R2.fastp-trim.20230215.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameE1_R2.fastp-trim.20230215.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24465103
Sequences flagged as poor quality0
Sequence length15-130
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAAAGAGTGTACGTCCTGGTGTAGATCTCGGTGGTCGCCGTATCATTA444470.18167509860882253Illumina Single End PCR Primer 1 (96% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)