FastQCFastQC Report
Thu 8 Sep 2022
Trueseq-stranded-mRNA-libraries-GeoRNA5-E1-NR005_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTrueseq-stranded-mRNA-libraries-GeoRNA5-E1-NR005_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51556
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2960.5741329816122275TruSeq Adapter, Index 5 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2230.43253937466056325No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC1000.19396384513926604TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)