FastQCFastQC Report
Thu 8 Sep 2022
Trueseq-stranded-mRNA-libraries-GeoRNA1-A1-NR006_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTrueseq-stranded-mRNA-libraries-GeoRNA1-A1-NR006_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3733527
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1326143.5519764555070847TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGC405241.085407980175314TruSeq Adapter, Index 6 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT137540.36839160397125825TruSeq Adapter, Index 6 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62380.16708061840720584No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56680.15181355324335408No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGCCGT45480.1218151094126278TruSeq Adapter, Index 6 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)