FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-larvae-day5-RNA-EPI-99-5_S40_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-larvae-day5-RNA-EPI-99-5_S40_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43415
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCCGTATCTCGTATGC1780.4099965449729356TruSeq Adapter, Index 21 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCCGTATCTCGTATG1730.3984797880916734TruSeq Adapter, Index 21 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAGTTCCGTATCTCGTATG1680.38696303121041115TruSeq Adapter, Index 21 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCAAGTTCCGTATCTCGTATGC1300.29943567891281814TruSeq Adapter, Index 21 (97% over 35bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC860.19808821835771045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)