FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-larvae-day5-RNA-EPI-99-4_S32_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-larvae-day5-RNA-EPI-99-4_S32_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305
Sequences flagged as poor quality0
Sequence length55-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC362.7586206896551726No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG60.45977011494252873Illumina Single End PCR Primer 1 (100% over 50bp)
CTAGTTTCCGGTGAGACACCAACAGCTCAACCAGGCCTTGTCTCTGGACA50.38314176245210724No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT50.38314176245210724Illumina Single End PCR Primer 1 (100% over 50bp)
CAGGAATCAACTTATGTGCTGGATGTGAAGGAACATGTGCAGAACTACCT30.22988505747126436No Hit
GTAATTGTGACTGCGCATGCCTTTGTTATAATTTTTTTTCTGGTGATACC30.22988505747126436No Hit
CTACCCTCTCTCCGTATTACAATTCTTCTTCTACTAGAGGTTCACAGTTA30.22988505747126436No Hit
GGAAGTAAACCGGTAAAAGGGTGCGATCATCTGGTTGTTATAAAAGGTTG30.22988505747126436No Hit
GTCCCATTGTTCTCTTCATGGCGATGAAGTGATACCATTGTGCAGACTGG30.22988505747126436No Hit
GGCAACATATTTTGTAACGTATTAAACGAGTCATGCATTTTCAAAATTTG30.22988505747126436No Hit
TCGAGCCCGCCAACCAGTTGGTTAAATGTGACCCGCGCCACGGTAAATAT30.22988505747126436No Hit
TATTCAAAATAGAATATTTCTTGATGCCACTGCTCTTATCTCATTTATTA20.15325670498084293No Hit
GCGGTCTCTCCAGTGGTGGTTTGTTAGTGAGTCTGTTAATGAGGTTTGCG20.15325670498084293No Hit
CCTGGTTCGAACCACTACTAAGCATATGCGGATAAAGTGTCTTGCTCAAG20.15325670498084293No Hit
CGGCAATTGAACTTTACAAGTTCTGTGTCACAGACACACAGGGACTTGGT20.15325670498084293No Hit
GTGATAACTCCAACACCAGGACCCAACCCCGTCTCTACAGAAAAACCCAA20.15325670498084293No Hit
TGCATGTTACTTTTTGTAGCAGTTGGATGATAGAGGATTTGATTACGTCG20.15325670498084293No Hit
CATAGCCACGCCTCACGTACACGTTACCCCAGCACCACACCTGACAGGCT20.15325670498084293No Hit
CATGTCTCTATATGTAGAACAGTACAAAATTAATTGGTGTGTGAGAAGTA20.15325670498084293No Hit
ACGCGTTCAATAGCAGACACTTATTATATAGATTTTCACATACCAGTAGT20.15325670498084293No Hit
AATACGTCGTTAACCGCCGACCCTGCAACTTGTACATGGTAACTGACGAC20.15325670498084293No Hit
GCAAAATTCAAGTGAAGTCTTTATATGTGTTTAAAAAAATGCACACTTCT20.15325670498084293No Hit
TACCGTGTGCGTGTTCGCCGTGGAGGACGTAAAAGGCCAGTACCAAAGGG20.15325670498084293No Hit
AGAGGGGTTCGGAATGAAATGAGGAAGAGGATTCTGCAAACACGAGATAT20.15325670498084293No Hit
CATGGGACCTGTTCTTGTTTGCTGATTTCAACAAGGATGGTTTTATTGAC20.15325670498084293No Hit
CTCAAATCCACAAACGAAAAGATACCCGAAGATGATTTCGTACGAAACAC20.15325670498084293No Hit
CCGTTAAACACTATTCATTCATTAATAACAGGATACATGAAATGACAAAA20.15325670498084293No Hit
GCCGGACAAGATGGCTTATCTGGCCAGGCAGGAAACCCAGGACAGAAAGG20.15325670498084293No Hit
TGGAACTCGCATCCTAAAAAATATGGTCCAGGGTCACGATATTGCCGAGT20.15325670498084293No Hit
TTACAAAGAACAATTTACTATGTAAATTAAATCTTTTTTATATTGCAAAA20.15325670498084293No Hit
TTTGTGTGTAAAAAATATCGTTTTGTGTAGATATAAATGTTGTTTTATTT20.15325670498084293No Hit
AATAGTTTAAAACAGCTTTCAAAAGTTAGTAATCTTAAGCATACAACAGA20.15325670498084293No Hit
CATTAATCAAAAACATGTAAATATTACCAGAGGAAAAACTTCAAATGAAA20.15325670498084293No Hit
GTGATATGCCACTGCGCCCGGTCACAGTATACTGACCCTGAACCTAGTTG20.15325670498084293No Hit
TCTGTTGGTAAAAGTGTTCAGAGAAAATCCATCATGGCTGATCAACTTAC20.15325670498084293No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCC20.15325670498084293No Hit
TTTTAATGTACGTTTTATATAGATTTTACTCTTGAATCACTTTTTATCTA20.15325670498084293No Hit
TTCACATAAAATGGAAGACCTTGTGAAATTGCTGTGCGGTATCAGCTACA20.15325670498084293No Hit
AGATGCTACCGGATAACATCGTGTCACGTGACCAAGAGAACGCATTCGGA20.15325670498084293No Hit
AAAATAGTTCACATCTTTTAGATATTCCTCCAACAGTTGACTGATGATTG20.15325670498084293No Hit
ATTTCATTCCTCGTATTATTTTAAGGCCATTTGTTTGTTTATTTGTTGGT20.15325670498084293No Hit
GGAGTACGTTTCAAGATTGTCAAAGTCGCTAACGTATCATTATTGGCCCT20.15325670498084293No Hit
ATTCAATGTGGATGTTTTTAGTGTCATTTAGTTTGTTAGGGTTAACTATA20.15325670498084293No Hit
GGATACAGTACGTGTGAAGAGGTCAAATATGACCCAACTTCCGGCAAAAT20.15325670498084293No Hit
GTTGACTTCTTTTGTTTATGCCCAATTTTATTTAAAATGACTAGTAAAAA20.15325670498084293No Hit
GCGGAGACCAAGAGGTCTGGATCCACCCAGTCGAGTGTGGAGACGAGTGA20.15325670498084293No Hit
AAGCTGTAATCGTCGTTATCAGATACTGGCGCCTCTTTCTGGACTTTTTG20.15325670498084293No Hit
TGAAAAAGTACCCTGTTTATTAAATACTTAGTTCATAAAACTGGTTTACA20.15325670498084293No Hit
TGGATTTGGCTTTGTCAGTTTTGAGGAACCAGAAGCTGCTGAGAAGGCTG20.15325670498084293No Hit
GTTCATACCAAACCAGCCTTTATATTTCTTCATTATTTGCTTAAATATAA20.15325670498084293No Hit
AGCTGGGGTTCATCCTGGGGACAAGAAGGTTATATCATGATGTCCAGGAA20.15325670498084293No Hit
ATTCCGATGGCCGAGGAGAAAATGAATGTTATGCTGGGTGACCCAGACAA20.15325670498084293No Hit
TTTGAATCCTCGCCCAGTCAGTCTCTGTGATGAAATATATGATGTATCTC20.15325670498084293No Hit
TTGAACAGCAAAGCAACAATACTAATAACAGATTGAGAATGAGTTGGATA20.15325670498084293No Hit
TGGCAGACTTTACTGCAAGGCTGTCTTCACAGGCTACAAACGTGGTCTCC20.15325670498084293No Hit
CGCAGCAATCCCACCAACTGTCGATACCCTCACACCCATTCCTGGTACAA20.15325670498084293No Hit
GAACTCTCAAATGTCGACAAATTCAGCACAATTTATCAGGAATTATGGAC20.15325670498084293No Hit
ATTCCCTACGTGGCTGACTTGTTCCAATACTCCATATGGGCAGAAACGTC20.15325670498084293No Hit
CAAAAAGACATTTTGGTTATTTCATACTTTTGTATATACGGTCTGTCATC20.15325670498084293No Hit
TCTAGTTTGGGCAGAAGTCCAGTTTATGCATAAATCTAGTTTGTGAAAAA20.15325670498084293No Hit
AGAACAGTGCGATGACCAGGGTGAGGCCAGTGGTATTGATGTTGTAATGA20.15325670498084293No Hit
AAATAAACTCATATTGAATAATTTCCGGATAATTTTTAAAATGAATTTAT20.15325670498084293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)