FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-larvae-day5-RNA-EPI-99-4_S32_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-larvae-day5-RNA-EPI-99-4_S32_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305
Sequences flagged as poor quality0
Sequence length55-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC403.065134099616858No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC251.9157088122605364No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCCGTATCTCGTATG50.38314176245210724TruSeq Adapter, Index 21 (97% over 39bp)
CCGGGCTGAGCTGTTGGAGTTTGTCCAGATACAAGGCCTGGTTTAGCTGT50.38314176245210724No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCCGTATCTCGTATGC30.22988505747126436TruSeq Adapter, Index 21 (97% over 38bp)
ACAACATGAACTATACACTACCTCCTGCATGCCACATGTATCTACTGCAT30.22988505747126436No Hit
TCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.22988505747126436No Hit
CCTCTCTCGACAAAAGCTGACCTTAAAAGTAACAAAACTGCCATAGGTAA30.22988505747126436No Hit
TCGGTTCCGAAGATGGCGATGGTGGACATTTACTGACATCCTCCCGTGAC30.22988505747126436No Hit
TGTTTTGATAGTTGCTATGGCAGCGTTGACGTCTTTCGGTACCACGTCAC30.22988505747126436No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.22988505747126436No Hit
AAAAGCGGAAACGGAAGACGCTGATATTATGATGGTCTGGTCTAATAATC30.22988505747126436No Hit
CCAATAGGAACTAACAACACGAAGAGCACCACAGCGGCGGCCGGCACGCT20.15325670498084293No Hit
GGGAGCATTTAAGTATGGTAAATACAAAAAGCAACAATTAAAAAATTTGC20.15325670498084293No Hit
GGGAACCGCAAACCTCATTTACAGACTCACCAACAAACCACCACTTGGGA20.15325670498084293No Hit
AAAACAACACCCACACGTAGTACAGTACCTGTTCCATACTACACTGGCCG20.15325670498084293No Hit
GTTTGCTGTGCTCCGACCCAGTATTTTCCATGTGGGCAGGTAGGAAGCTG20.15325670498084293No Hit
TAGTACAGTATTCTAAGTAATAACCAATTAACCATTCGCATCACTTGCTT20.15325670498084293No Hit
GTAAATTCAAATTTAGATTATGTAAAATTAAAACTGCAGCGTGTCTTTTT20.15325670498084293No Hit
GTTTTCCCACATTTGGTTTTCCCTATATTTAGATGTACTGTATAGTAACA20.15325670498084293No Hit
CTGCAATTAGATATATAATGATAATAGTGTTTGTCTATGACAAAGAAGTA20.15325670498084293No Hit
CTCAGTCCATCCTCACCAGAATGTTTATAAATTGTTTTCATTTGGAGCGA20.15325670498084293No Hit
CACAGACCTTGAACATTATCAGGGTCATTCCAAGGCTGTCTGACAGCCTC20.15325670498084293No Hit
TACACGGAGGCCTCTACTGCCAAACACTGCTCCCATGATCCGAGCCATAC20.15325670498084293No Hit
TCTGTATGCACTAATATCTGGCTCTGCTGTCTTGGTATCTCCCATTGGAG20.15325670498084293No Hit
CTGCTCCAAAAGATAGAGACTCAGGCAAGACTTGTATTTACATCAACACT20.15325670498084293No Hit
CACGTATAAAGGCTTGGCCACCACGATTCTACCGTTCATCTCTGTCACGG20.15325670498084293No Hit
CCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.15325670498084293No Hit
ATAAATACTACAAATCTTGAGAAAAAAAAGCAGATTGACATTTCAAACAA20.15325670498084293No Hit
TCGCATTACAGTTCCCAATTCTTTGGTTGTAATGGTACCATCTCCATCCT20.15325670498084293No Hit
CCTAGACCTTATTTGACATAGAACATAGAACATATTTCAACTGTGTATAT20.15325670498084293No Hit
AAAAGCTAGTCAAGTTGAGTTTGCAAAATTCACTATTGATTTATTATATC20.15325670498084293No Hit
GGACGGCGCAGATCACGACATTTAGGATCAACCAAGTCCCTGTGTGTCTG20.15325670498084293No Hit
CGTATTGTATTTTAAATACAACTCACTTTTTCAAAGTCAAGACCTAAGAA20.15325670498084293No Hit
CTAGATTTAAGTACAAACTAGACTTCTGCCCAAACTAGACTTCTTCACAA20.15325670498084293No Hit
GTTCAGTGGTATAGGGTAAAGTGCACTTTTAGGATTTTCGATCTAAATAC20.15325670498084293No Hit
AACAACAATAAAAATAATACTATGTAAAACAGCAGAATTTGTCATGTTCA20.15325670498084293No Hit
CTGCATTTTCGGGTATATAAACTGTTGTATCGTTTTCATTGAACAAATCA20.15325670498084293No Hit
GTCCATATTTAAGTGTTTTGTAGCTGTGACAAGAAATGAATAAATAAAAT20.15325670498084293No Hit
ATAAAGTTGATTAATAACATAAAGTTTAGGGTTGTTTATTTTTTAAAAAA20.15325670498084293No Hit
TTTGGATCATATAATTTTCAAAAATAGTTTTAAACGAAAATTATCAAAAT20.15325670498084293No Hit
GTAGCTTTCAAGAAAACAATTTTGTTGTTTTTTACAAATATCACACTGAA20.15325670498084293No Hit
GTGATCTGTTCAGTGATAAAGTGGTAGAGCAACGACTGTGCGTTAGGAAA20.15325670498084293No Hit
CCACAATAAGCCATGCAGTTCTGTTTTGCCCAAGTTTCATACGGAGCTAC20.15325670498084293No Hit
GCTCCGGTCTTGCCGTCATCACAGATACAGATCTGCTGACATCCATCGTA20.15325670498084293No Hit
TTAACAGACACTTGTTAAAAATTTACAGACTAAGCACTTATGGTCAAGTA20.15325670498084293No Hit
TTTTTTAGGGCCAAATCTTTATTTTGCCAATATGTACAGTTTAAGACCTT20.15325670498084293No Hit
CCGCGGTCTCCCTGTCGGCCCTGTCCTCCAAGGGGTCCTGGTGGACCGGA20.15325670498084293No Hit
TCTTCATCTGCTTCCTCAAAAGACAGTGATCCGTCTTGCTCTTCGTCCTC20.15325670498084293No Hit
CAAATAACAAGAATTACCGTTAAACATAAGCTGAATCTATCCTCTCAACC20.15325670498084293No Hit
AGCCAGTGCAATAATCCACCTTGGTAATTGCAAATAACTTTTTTTCCAAT20.15325670498084293No Hit
GTTGTCTTTAGCCCATCCGGCATAATCGTTGCATACAGCTGGGATTTCAA20.15325670498084293No Hit
CTAGTAGTTGATGATTAGCGTCTGAATCTAACGTATTTCTCGATGCCCGG20.15325670498084293No Hit
ACGCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.15325670498084293No Hit
CTTTAGTCAAGCTTTTTGAAGCCAATGTCATCACTGTACTCTCTAAAACA20.15325670498084293No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.15325670498084293No Hit
AGAGAGAAAAAATAAATCGAAACGTAGTGAATATCTAAGAAATAGATATT20.15325670498084293No Hit
CAACTTTCAGTTTTTTCATTTTTTCTTCACATATTCTCTTGATTTGCCTG20.15325670498084293No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCAAGTTCCGTATCTCGTATGC20.15325670498084293TruSeq Adapter, Index 21 (97% over 35bp)
TTTTTTTCTGTGCACAGAGGTTTTATTAAATAAATATACACAGGAACAAT20.15325670498084293No Hit
GAACTTTCTCCACAGTTGCAGGAACATCAAATGTAAAATATCCTGTCTGT20.15325670498084293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)