FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-larvae-day5-RNA-EPI-99-2_S16_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-larvae-day5-RNA-EPI-99-2_S16_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC816.672158154859967No Hit
AATCAATTCCCAATACCACCGACGTTTATTGGAACTCCTAGTTTTACAAA50.41186161449752884No Hit
TGATGCATATTTCTTTTATAATATAGTTTCTAGATGAGGGTCTTTAGTTT40.3294892915980231No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT40.3294892915980231Illumina Single End PCR Primer 1 (100% over 50bp)
TGCACATTTTAGTATGACGTGCTATTCTCAAAAAATGGTTAATGAGTGTA40.3294892915980231No Hit
TCCCTCTGTGAGCAGACAACATAAAAAAATGTTCTCTGTGGCTGACAACA30.2471169686985173No Hit
GAATGGCTTAATTTCTAAATCACTGAATAAGTAAATTAATGGTCATTTCT30.2471169686985173No Hit
CTGTGACACGTGATCAACACATGTCTGCAGACGTCAGTCTTACGTAATCA30.2471169686985173No Hit
GGACAATTTGACTTTCACATATTTATATCTCTTCCAATAAAATATCATAA30.2471169686985173No Hit
TTTGAAGCCACATTAGACGAAAAATGCTCGAGTCACGTGATCATTTTAAG30.2471169686985173No Hit
GGGTAGGTATGTATCTGCATGTATGTATGTATCTGTTTGTATGTATATAA30.2471169686985173No Hit
GCTCAGTGTTTTTATTGAAATTGTATTATTGTTGAATGAAAAGATTTATC30.2471169686985173No Hit
AGTGGACTACTTGTCTATTTGATGTTAGAGATAGATTTGTTGATGAAATA30.2471169686985173No Hit
CTTCATCTGGCGGGGGTATCTTCTATTTTGGGGGCTATTAATTTCATTTC20.16474464579901155No Hit
CCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.16474464579901155No Hit
TAAAGACAATGATGAGGAAATGGTTACCAGCTGGTGATGCCTTACTCCAGATGATAGTCATTCATCT20.16474464579901155No Hit
GTTAAATTAAGTGATGCTGGGCATTATGTTTCAACAGTTGGGCATTACAA20.16474464579901155No Hit
AGAATTGTTGCCTACATCTGGCAACAGAGCATGTACATCTGTTGTCATAG20.16474464579901155No Hit
CCCCCCCCCCCCCCCCCCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCC20.16474464579901155No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCC20.16474464579901155No Hit
GTGTCATTTTACAATTCTTTACTAGTGTAGAGTAACGGTGTTCATTTAAA20.16474464579901155No Hit
TGGTTCCGCCCCTGGGACCCATTATAGAATATGTATACACCAATATTTGT20.16474464579901155No Hit
TCAATTGGTGCATGTGCATTGTATTTTAAAGTTACAACAGATACTGTTCT20.16474464579901155No Hit
GTTTTATTTATAAATACTAAAGAGTCCATTAATTATTTATTTATTTAATT20.16474464579901155No Hit
CTCAAATAAATTAGTATAAACTAATAATCTTAGTCGAAGATACATCGGCC20.16474464579901155No Hit
ATTTTATGAACGCTCGCGTACTCAGGTAAAATCTCGCGAAATCTCGTTTT20.16474464579901155No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCC20.16474464579901155No Hit
TCCTTACAGTGTTTGCCGTCGTCTGCCTTCTGTCGCAGACGGTAGTGTCG20.16474464579901155No Hit
GTAGTGATAATGATATTGTTGATGATACTGGAAGTAATGGTGAAAGTGTT20.16474464579901155No Hit
GCATAACGCAACATGGAGTAATCTTCAGAAGACATGTCGATTAGTCGACA20.16474464579901155No Hit
CCAACGAGCACGACTACAGTACCAACGACCACGACTACTGTACCAACGAC20.16474464579901155No Hit
CAAACATGTCCGTGCCACAGTACAACCAGTTTGGTAACATACAGAACGGA20.16474464579901155No Hit
CCACAGATGAGGGCTGGACCTCGCTGGCAGACAGTACGACAGCCACAACC20.16474464579901155No Hit
TGCCGTTCGTGTAAGCTCTGAGCCAGTGCTTTGTAATAAAGCTAAAATAA20.16474464579901155No Hit
CTCCACCCTTCTGGAGTACAGAGTATAGATAGATTACGTCTCTGTCATGA20.16474464579901155No Hit
CCTTGGTGGCGGTACCGGATCTGGTATGGGAACACTCCTCATCAGCAAGA20.16474464579901155No Hit
CACATCTGCTATGCAGATAGCTTTTTAAAAATTAGTTATCTATGTCTCCA20.16474464579901155No Hit
GCAAAATAATACAAAATGGGAGTCGATTACTACAATATTTTACAACTAAC20.16474464579901155No Hit
CCCAGAATGAACTGGATGCCCAACTGGCAAGGTTGGAGAAAACATATAAT20.16474464579901155No Hit
CTCGCGAACAAAAGACAATTTTCAGGATGCATAACCTGCGTTCTATAAGT20.16474464579901155No Hit
CCTTATATCAGGGTCTTGGAAGACAGAGTCCAAACTTGTTTCAAAAGAGT20.16474464579901155No Hit
GTTGATAGCTCTTCACCATGTAAATGATTCATTGAAGTTGTATAAATGTG20.16474464579901155No Hit
GACACAGTTTGGTACCGACGAGACTGAAGCACAGTGTGAACAAACACTCT20.16474464579901155No Hit
TAACCACAGTCAAGAAAGGCAAACCAGAACTCAGAAAAAAAGTTATGCCT20.16474464579901155No Hit
CGGGATAAGTCTGGTCTACTGGATCTGAATGCCATCAAGCAGGTGATATC20.16474464579901155No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG20.16474464579901155Illumina Single End PCR Primer 1 (100% over 50bp)
TCTTTATGTAACATCATGCACTCGATAAAATGTGAGTTAAATATTGATTA20.16474464579901155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)