FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-8_S63_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-8_S63_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20155
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3441.7067725130240636Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT980.486231704291739Illumina Single End PCR Primer 1 (100% over 50bp)
NATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG910.45150086827090047Illumina Single End PCR Primer 1 (98% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.38700074423220043No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC620.30761597618456954No Hit
CTTCTTAAGCCACCCACCATGTTTCATTACAGAAAAAAATTGAATTATCG320.15876953609526173No Hit
CTTCTTAATAACTAAGAACGAACTTAATAATTAAAGTACGTGTAGGCTGC310.15380798809228477No Hit
CTGCGCCATCCGATCTGCCGAGTCTTCTCTGTTTAGCAATGGCTGGTGTT310.15380798809228477No Hit
TGAAGGTTGTGTCTATATTGGGTGTGGCCATAAAACCTCTCCACAGAAGA310.15380798809228477No Hit
TGTATTTCTGTACATTTCATGAAGTATGGCATCAAATCTCAGAAGACAGT310.15380798809228477No Hit
TGCCAATTCACAAAGCGCGAACACTTATGAACTTTTATATCACATAAATT300.14884644008930786No Hit
GCCATATATTCTTAATAAACAGATAAGTGTTTGCTTACAAGTGTTATTAT290.14388489208633093No Hit
CTTGGATCAAGATGCGAGCCAAGTGGAGAAAGAAGCGTATGCGCAGGTTG280.13892334408335402No Hit
CAGTTTAATGATATTGCACGGTGACAATTGATTGTTAAGCTCAAGCAGTG270.13396179608037706No Hit
CATCCCTGGTCTAGATTTCTTACAGGGTCATGTCCTTGGTTTCTTTGATT260.12900024807740015No Hit
CCGGGAGCACGGTGGTTCTATTCAGATTCATTTTGATGTATTTTTCCGCC260.12900024807740015No Hit
GGAAAGAAGAGTTTTTATATGTTTTAAAGTGTTAGGGGCGTGACTTTTAT260.12900024807740015No Hit
TGGAAGAGTATATAGGCGCATCTTTTTCTATTTCGGATGGGGTGTATGGC260.12900024807740015No Hit
GGACACCCGCCTTAACAAACATTTATGGTCACAAGGAATCAGAAATGTTC250.12403870007442323No Hit
GGTCAAGCTTGCTGTACTCAAGTACTACAAGGTTGACGAGAATGGCAAAA250.12403870007442323No Hit
TTCAAAACTACCGCTCCAACAGGAGTGATGTCTAACAAGTCATTTGTGGA250.12403870007442323No Hit
GCCAAATATCGAACTTGACCTATATTTTATGATAAGACACACAAAATATA250.12403870007442323No Hit
CAGTAACACATTGTATGAATTATTATGCTCCCGCAAAGGAGGGAAGCATA250.12403870007442323No Hit
TGGCTTCTATCTGCCAGTGTGGTTACTTTAATCGGTTGACTTATCACTCC240.11907715207144628No Hit
CTTTGATTTAGCATGATAAAACAAGAAAACAGAAAAAAGAAAGATGTAGA240.11907715207144628No Hit
CTTACTAATAGTGGAAGATCAAAAAAATTGAACCATTTGTCTCCCTCCCA240.11907715207144628No Hit
CAGATGTGAAAGAAAATTTTAAAGAGGCCGCCCCATCCACATCCTTTGGT230.11411560406846936No Hit
TTAAGAACATTGGAATCATGCAGCATGTGATATTAAAGAACCAACTTTAA230.11411560406846936No Hit
TTGTAAGCTAACAGACAAAATTATAAATACAACCTGGATCCTTTGGTAAC230.11411560406846936No Hit
TGAAAACCTACAAAATAAATTTAATTCAAATTCTAAAAACAAAACAATAA230.11411560406846936No Hit
AAACACGCTCGTTTATCGTTACCACCCCGTCCAACGTATACGTTACATAA230.11411560406846936No Hit
TCAACATTATGCACTGCGTCTTCAGATATTTTTTCAGCATATGTCAGCTG230.11411560406846936No Hit
AAAAATTTAACAAGATTTAGACTGAGTGCGCATAATTTAGAAATTGAAAC220.10915405606549244No Hit
CCAGACTCCTGAACCGACACTTCCAAAAATTCTGCGCCGAGTGATTCTGC220.10915405606549244No Hit
GGGCTTCTTTGACTATAAAGAATTTAGTTCTTTATCATAATTTGGCTTTA220.10915405606549244No Hit
GTACCATTCACCTTGGTAACAACCTAGAAATCTACTGCGGTAACCATGAC220.10915405606549244No Hit
GCCTTTCCTTCTCTATATAATTCCTTGATTTAGTGTTGTCTATATAGTAA220.10915405606549244No Hit
GATTGTCAAAAAATTGACAGATGCTGATGCGATGAAGTACACAATTGTTG220.10915405606549244No Hit
GTTTTTAGATTCCCTTATAACTTGCCTAGCTCAAAAACATAAGGTGAATG210.1041925080625155No Hit
GGAACAAGCAACAGCCCTTCTCATGGCAGGGAGACATCTTCCAGAGTCCC210.1041925080625155No Hit
AGTAAATATCACTTTGAAAATGGCAGATTTCTTTATGCTGTATGCAAGAT210.1041925080625155No Hit
CTGCATCTTTGCAGTGTATGATCCAGTCAAATGTCACATCTTCTTCTAGA210.1041925080625155No Hit
CTAACATTTATATATATAAAATGGGCAAAATAGTTAAATAATTCAGAATC210.1041925080625155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)