FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-8_S63_L008_R2_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-8_S63_L008_R2_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18021
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.42727928527828646No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC620.34404306087342545No Hit
CTTCTTAATAACTAAGAACGAACTTAATAATTAAAGTACGTGTAGGCTGC310.17202153043671273No Hit
CTGCGCCATCCGATCTGCCGAGTCTTCTCTGTTTAGCAATGGCTGGTGTT310.17202153043671273No Hit
TGAAGGTTGTGTCTATATTGGGTGTGGCCATAAAACCTCTCCACAGAAGA310.17202153043671273No Hit
TGCCAATTCACAAAGCGCGAACACTTATGAACTTTTATATCACATAAATT300.166472448809722No Hit
TGTATTTCTGTACATTTCATGAAGTATGGCATCAAATCTCAGAAGACAGT300.166472448809722No Hit
GCCATATATTCTTAATAAACAGATAAGTGTTTGCTTACAAGTGTTATTAT290.16092336718273126No Hit
CTTGGATCAAGATGCGAGCCAAGTGGAGAAAGAAGCGTATGCGCAGGTTG270.1498252039287498No Hit
CAGTTTAATGATATTGCACGGTGACAATTGATTGTTAAGCTCAAGCAGTG270.1498252039287498No Hit
CATCCCTGGTCTAGATTTCTTACAGGGTCATGTCCTTGGTTTCTTTGATT260.14427612230175907No Hit
GGAAAGAAGAGTTTTTATATGTTTTAAAGTGTTAGGGGCGTGACTTTTAT260.14427612230175907No Hit
TGGAAGAGTATATAGGCGCATCTTTTTCTATTTCGGATGGGGTGTATGGC260.14427612230175907No Hit
CCGGGAGCACGGTGGTTCTATTCAGATTCATTTTGATGTATTTTTCCGCC250.13872704067476832No Hit
GGTCAAGCTTGCTGTACTCAAGTACTACAAGGTTGACGAGAATGGCAAAA250.13872704067476832No Hit
TTCAAAACTACCGCTCCAACAGGAGTGATGTCTAACAAGTCATTTGTGGA250.13872704067476832No Hit
GCCAAATATCGAACTTGACCTATATTTTATGATAAGACACACAAAATATA250.13872704067476832No Hit
CAGTAACACATTGTATGAATTATTATGCTCCCGCAAAGGAGGGAAGCATA250.13872704067476832No Hit
TGGCTTCTATCTGCCAGTGTGGTTACTTTAATCGGTTGACTTATCACTCC240.1331779590477776No Hit
CTTACTAATAGTGGAAGATCAAAAAAATTGAACCATTTGTCTCCCTCCCA240.1331779590477776No Hit
GGACACCCGCCTTAACAAACATTTATGGTCACAAGGAATCAGAAATGTTC240.1331779590477776No Hit
CTTCTTAAGCCACCCACCATGTTTCATTACAGAAAAAAATTGAATTATCG240.1331779590477776No Hit
CAGATGTGAAAGAAAATTTTAAAGAGGCCGCCCCATCCACATCCTTTGGT230.12762887742078685No Hit
TTAAGAACATTGGAATCATGCAGCATGTGATATTAAAGAACCAACTTTAA230.12762887742078685No Hit
CTTTGATTTAGCATGATAAAACAAGAAAACAGAAAAAAGAAAGATGTAGA230.12762887742078685No Hit
TTGTAAGCTAACAGACAAAATTATAAATACAACCTGGATCCTTTGGTAAC230.12762887742078685No Hit
TCAACATTATGCACTGCGTCTTCAGATATTTTTTCAGCATATGTCAGCTG230.12762887742078685No Hit
CCAGACTCCTGAACCGACACTTCCAAAAATTCTGCGCCGAGTGATTCTGC220.12207979579379612No Hit
GGGCTTCTTTGACTATAAAGAATTTAGTTCTTTATCATAATTTGGCTTTA220.12207979579379612No Hit
GCCTTTCCTTCTCTATATAATTCCTTGATTTAGTGTTGTCTATATAGTAA220.12207979579379612No Hit
GATTGTCAAAAAATTGACAGATGCTGATGCGATGAAGTACACAATTGTTG220.12207979579379612No Hit
AAACACGCTCGTTTATCGTTACCACCCCGTCCAACGTATACGTTACATAA220.12207979579379612No Hit
AAAAATTTAACAAGATTTAGACTGAGTGCGCATAATTTAGAAATTGAAAC210.1165307141668054No Hit
GTTTTTAGATTCCCTTATAACTTGCCTAGCTCAAAAACATAAGGTGAATG210.1165307141668054No Hit
GGAACAAGCAACAGCCCTTCTCATGGCAGGGAGACATCTTCCAGAGTCCC210.1165307141668054No Hit
AGTAAATATCACTTTGAAAATGGCAGATTTCTTTATGCTGTATGCAAGAT210.1165307141668054No Hit
CTGCATCTTTGCAGTGTATGATCCAGTCAAATGTCACATCTTCTTCTAGA210.1165307141668054No Hit
TGAAAACCTACAAAATAAATTTAATTCAAATTCTAAAAACAAAACAATAA210.1165307141668054No Hit
CTAACATTTATATATATAAAATGGGCAAAATAGTTAAATAATTCAGAATC210.1165307141668054No Hit
TGCTAATTTAACTATTTTGTGTTATATATTTCTGTTGCTTTATAATTATA200.11098163253981466No Hit
CATTTAATACATAATTTAAGGTTTTCATTCTCATTTTAAAACAGTGCTTA200.11098163253981466No Hit
ATTTCTGCAGCCAAAGTATTCTGTGGTATGGATGAAATATTATTATGCAA200.11098163253981466No Hit
AGTGTTAATAGTTAACTGACATTGTTCAACTCGGTCATCATAAATCCGAT200.11098163253981466No Hit
CTCGTTTAGAAGCTGCTGAAGCCAAAATTGTGGAGCTTAGTGAGGAACTG200.11098163253981466No Hit
CAAAAAGTAATAATTTTGCGACAGTAAAAGTGTTAAAGAGACTATTAAAC190.10543255091282391No Hit
GGCAACTTAGATTGGATTAACAACGCAGTTTCGACTTTGTTTACTGTATC190.10543255091282391No Hit
GTACCATTCACCTTGGTAACAACCTAGAAATCTACTGCGGTAACCATGAC190.10543255091282391No Hit
GTTTAGTACAGTTATTAAAAAAGTAATTTTATTTTTAATTTCATACAAAT190.10543255091282391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)