FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-8_S63_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-8_S63_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20155
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2211.0965021086579014TruSeq Adapter, Index 6 (100% over 50bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1210.6003473083602084No Hit
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC830.4118084842470851TruSeq Adapter, Index 6 (98% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.30265442818159266No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC580.28776978417266186TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGCAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC500.24807740014884647TruSeq Adapter, Index 6 (98% over 50bp)
TATGTGTTATTACAAATAATAATAAATATTCTATTAAAAGCTATATAACT310.15380798809228477No Hit
TAATATAATACAACCATCAATTTCCATTTTCAAAAAGAAAAATGAAAACA310.15380798809228477No Hit
CGACACACAAGGAGTATCGTTTACTTTTGTGAAGACAAACATTTTGTTTC300.14884644008930786No Hit
CCTCCGCAAAGTGATTCATGTAAGTCCGAACTAACCCAACTAGTAAAAAT290.14388489208633093No Hit
TGTAAGAAATAACTCAAACACACATATTGTATAGTAGCACTAAGAGAATT290.14388489208633093No Hit
TGAGGTTTCACTGCTTTCATCAGAATGAGACCCTGCGTTGCTTAGATGCC270.13396179608037706No Hit
CACCTTCATAGTAGCCATTTTGATCCACCGACCCTGTAACTGTGACAATA260.12900024807740015No Hit
CAAACACTTATCAGCAAACATTTGTATGCTATTATCTGTCAGCAAAAACT260.12900024807740015No Hit
CTCCGTGAAAAATCATCGAATGGGAACACAAAGACAATATGCACATCTGC250.12403870007442323No Hit
GGAAGTCTGAACTCTGGTGAATGTCATTAGTCGTTTTGTGCAATGAAAGT250.12403870007442323No Hit
GGAAGCGACGCCAATCTACATACTCATTGTCGGACGATGCTCCCGTCGCT240.11907715207144628No Hit
AAATTGTTCAAAATGTTACTTGCTTGAGCTACCTTTATCAACACCAATAT230.11411560406846936No Hit
AAACTATTCAAATCGTACAAGTCAATATATTATTATAACAGAGCTGTTCG230.11411560406846936No Hit
GGGAGAGTTCCACGAAATACAAAATCAGATTCAATATATTTGTCATTTAC230.11411560406846936No Hit
GCAAAACTTTGTTACGCTTCTGATTTACTTCAACTTGAATTAACATTCCA230.11411560406846936No Hit
GTCGTGTACGATTTCTTCTGCAACACTTTGAGTAAACAAGACTGTCCAGT230.11411560406846936No Hit
CAATTTTGTTGCCACAGCAACCACAATTTTTGTCCGATTGTAAAAGATGA230.11411560406846936No Hit
CCTAAGAATAAATTGTTTGTGCTTTATGAATTTTGACTAGAAAGGATTGC230.11411560406846936No Hit
TTTTTCTTTACCACAGTCTTTAATTCACTAAACAAATCACAGAACACTAA220.10915405606549244No Hit
GGACAACATAATAGAAAATGATACTCCGATTCAACCAACCTCCCATTACA220.10915405606549244No Hit
TTTTAATCCTAAAAATCAGATTTATTTGTTAAGACAATTTTAAAACACAA220.10915405606549244No Hit
GTTTTATTTACTATTTGATAGATATGGAAAGGCTATAACTAAACAATGTT220.10915405606549244No Hit
TCACATTCTACCACATTCACCTAATGTCTTTGATCAAGGCACCTTATAAG210.1041925080625155No Hit
CTCGGTTGACATCTCCTTGATATCCTTGGCGTAACCACCAGCTGCCGCGC210.1041925080625155No Hit
ACCTGGTCATACACTGCAGAGATACAGTCTAGAAGGAGATGTGACATTTA210.1041925080625155No Hit
ATCAACTCCATGTTTTCGTAACAATAACATGCTAGTAATCGGGCTAGAAT210.1041925080625155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)