FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-8_S63_L008_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-8_S63_L008_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18021
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC760.42173020365129565No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.3384939792464347No Hit
TATGTGTTATTACAAATAATAATAAATATTCTATTAAAAGCTATATAACT310.17202153043671273No Hit
TAATATAATACAACCATCAATTTCCATTTTCAAAAAGAAAAATGAAAACA310.17202153043671273No Hit
CGACACACAAGGAGTATCGTTTACTTTTGTGAAGACAAACATTTTGTTTC300.166472448809722No Hit
CCTCCGCAAAGTGATTCATGTAAGTCCGAACTAACCCAACTAGTAAAAAT290.16092336718273126No Hit
TGAGGTTTCACTGCTTTCATCAGAATGAGACCCTGCGTTGCTTAGATGCC270.1498252039287498No Hit
CACCTTCATAGTAGCCATTTTGATCCACCGACCCTGTAACTGTGACAATA260.14427612230175907No Hit
CAAACACTTATCAGCAAACATTTGTATGCTATTATCTGTCAGCAAAAACT260.14427612230175907No Hit
CTCCGTGAAAAATCATCGAATGGGAACACAAAGACAATATGCACATCTGC250.13872704067476832No Hit
GGAAGTCTGAACTCTGGTGAATGTCATTAGTCGTTTTGTGCAATGAAAGT250.13872704067476832No Hit
GGAAGCGACGCCAATCTACATACTCATTGTCGGACGATGCTCCCGTCGCT240.1331779590477776No Hit
TGTAAGAAATAACTCAAACACACATATTGTATAGTAGCACTAAGAGAATT240.1331779590477776No Hit
AAATTGTTCAAAATGTTACTTGCTTGAGCTACCTTTATCAACACCAATAT230.12762887742078685No Hit
AAACTATTCAAATCGTACAAGTCAATATATTATTATAACAGAGCTGTTCG230.12762887742078685No Hit
GCAAAACTTTGTTACGCTTCTGATTTACTTCAACTTGAATTAACATTCCA230.12762887742078685No Hit
GTCGTGTACGATTTCTTCTGCAACACTTTGAGTAAACAAGACTGTCCAGT230.12762887742078685No Hit
CAATTTTGTTGCCACAGCAACCACAATTTTTGTCCGATTGTAAAAGATGA230.12762887742078685No Hit
CCTAAGAATAAATTGTTTGTGCTTTATGAATTTTGACTAGAAAGGATTGC230.12762887742078685No Hit
GGGAGAGTTCCACGAAATACAAAATCAGATTCAATATATTTGTCATTTAC220.12207979579379612No Hit
TTTTTCTTTACCACAGTCTTTAATTCACTAAACAAATCACAGAACACTAA220.12207979579379612No Hit
GGACAACATAATAGAAAATGATACTCCGATTCAACCAACCTCCCATTACA220.12207979579379612No Hit
TTTTAATCCTAAAAATCAGATTTATTTGTTAAGACAATTTTAAAACACAA220.12207979579379612No Hit
GTTTTATTTACTATTTGATAGATATGGAAAGGCTATAACTAAACAATGTT220.12207979579379612No Hit
TCACATTCTACCACATTCACCTAATGTCTTTGATCAAGGCACCTTATAAG210.1165307141668054No Hit
CTCGGTTGACATCTCCTTGATATCCTTGGCGTAACCACCAGCTGCCGCGC210.1165307141668054No Hit
ACCTGGTCATACACTGCAGAGATACAGTCTAGAAGGAGATGTGACATTTA210.1165307141668054No Hit
ATCAACTCCATGTTTTCGTAACAATAACATGCTAGTAATCGGGCTAGAAT210.1165307141668054No Hit
GGCGTCCTTAAGTCTCTGAGTCAAGTCACGGATCGTCTCTTCGTAGCTGT200.11098163253981466No Hit
TTCGAAACATATTTATATGCATATAACAAACAGCGAAGTAGAAATATATC200.11098163253981466No Hit
CTCCACACATTCATAACTGTCCATACTTGACGCCATCATCGCTGGTGGCG200.11098163253981466No Hit
GTGACAACTTGTCCCGAACTCATGTTTCGGAGTCATCTTGTAGTGACTGC200.11098163253981466No Hit
CTGTAGGCAACGGCCTGTTTTGACAAATCATCATAGATGATGAGGGCATG200.11098163253981466No Hit
GGCTTTGTCAAAGGTTCCGTCAATTTCATTACATTCTTATAATTGCCACT200.11098163253981466No Hit
TCCCTTCATAAACCTATCAGCCCTATAAACATAATCGTACCTTCAATATC190.10543255091282391No Hit
TATTGGTTAATACCAGTAATTTTATAACTTAAGCATATATCTTAACAACT190.10543255091282391No Hit
TTTTATTTGTTACTGTACACTTTATTCATTATCCACATTTATGGTCTGGG190.10543255091282391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)