FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-7_S55_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-7_S55_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC160.8346374543557642No Hit
CTGACACCCGCTGAAGTGAAGGACTACCTGGCATCTGTACAATGATCTGT50.2608242044861763No Hit
GTTCCTAGCAAGGACTGTCACTGTTATATAAGAGTCCCTAGCAAGGACTG40.20865936358894105No Hit
CTTTCCCTTAATAAAATACCAAATAGTAGTTAAAGAACATAGCAGTAGTA40.20865936358894105No Hit
CGACATTGTGTCTCATTATTATTCGTCTCTCACCACTGATTCAAGTGGGA30.1564945226917058No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30.1564945226917058No Hit
TAGAAGGATAACATCATTTAGTTTCCTCCTAATCAAGGTTAATGTATGAG30.1564945226917058No Hit
CTTGATTATGATTTTGAGATAATGGGTGTCATTTTTACAAGTCTTTACTA30.1564945226917058No Hit
CCCAGCAACCGTTGCAGAGTTTTACATGTAAATAAGAATGTGAAGATTGA30.1564945226917058No Hit
GTTACCTGCCCGTCAGTTAATGTGATATTATTGCTAAAGAGCATAAAATA30.1564945226917058No Hit
GGTAACTTATCATTTGACGACATCTTGAAGATTGCCCGCACAATGAGGGA30.1564945226917058No Hit
ATTGAAATGTTTTTGATATGTTATGCTTGTTTGTGAGGTATACACAAGTG30.1564945226917058No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG30.1564945226917058Illumina Single End PCR Primer 1 (100% over 50bp)
ATTAGATAAAAGTTCATTTGAAGACAGTCCATCCATTTACAGTATTTGCT20.10432968179447052No Hit
ATATCGAGGAAGACCAGAATGATTGATGTTGTATACAATGCTAGTAACAA20.10432968179447052No Hit
CGTTACTGTAAGGACTATCTCTCTCTCTGTCTGTAGGATTTTATTAATAA20.10432968179447052No Hit
CGGCCGCAGTTAGAAGCTGTGCTAAGGTAGCGTAATAAATTGTCTTTTAA20.10432968179447052No Hit
CTTTAACTTACATATTGAAATATCTACGTCTACTGTATTGATGATTGTTG20.10432968179447052No Hit
GGCCAATGCCTATATAAAAGTCGAAGGACGTTTCCACTACAAGAAGTGGT20.10432968179447052No Hit
ATAGCAAATACATATATTCGATATACAGCGATATATAACAACAGAAGTGA20.10432968179447052No Hit
ATCATGGAAGGAAAGGTCACTCTCGATTCCTTGAAGATCCATGCTGCTGC20.10432968179447052No Hit
TTGGAATTTTAATCTCAAATATTATGAAATCAGTAAATAAAAGCAGGATG20.10432968179447052No Hit
TGTGATTCACGACTTTCAGAGGAATGTGACGGTGATAGTGGACAATGATG20.10432968179447052No Hit
TGGAATTGAGCATCAACCAACAAGTTGTATACATTAATAAATCGAGTTCT20.10432968179447052No Hit
AGTCTACGTGTTGGCCATCTTTTGGAAACGGACGAACGAACAGGGCGCAT20.10432968179447052No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10432968179447052No Hit
TGTGGATCAGCAAGCAGGAATATGACGAATCCGGCCCATCCATCGTCCAC20.10432968179447052No Hit
CTTTCGTCGGTCGGATAGAAGATCGCATGCCAGTGTTACATATTGTTCTC20.10432968179447052No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCAC20.10432968179447052No Hit
CAGGAATTAAAGTATTTAGTTGAATTGCTACAATCAGAGGAAGGAATGTA20.10432968179447052No Hit
TCCGCTTTTGAGTGCTGCCATATTGGATTTGCTGTTCTGTCATACATGTG20.10432968179447052No Hit
CTGATATCTATATGTATGTTGTTGTTCAATGCATTGAAGTGCTGCTTCCT20.10432968179447052No Hit
CTGAAGATATGTTTCAAGTCCCTAGAGAATTAGTAGCCAAAATTATTGGA20.10432968179447052No Hit
TGAAGATGAAGGGTGTAAATCATTAATGAAGCTGTTCTGTACAAAGTCTC20.10432968179447052No Hit
GTGTTATTGAAATTTGTGATATTTGAGGATTATTTTTATCAGGAATAAAT20.10432968179447052No Hit
CTGCTCGTGTTATCATCGAGAAGTATTACACTAAGCTGACCCAAGATTTC20.10432968179447052No Hit
TTCAAACGTAACGCACACACGCCAGAACTTAATTTTCATCAAAACACAGC20.10432968179447052No Hit
TGGAAATAAACGACTTTCTTATGGAAAAGCTTGTACAGTAAACATCTTAA20.10432968179447052No Hit
CAGGTCTAAGATCAAGCCTTTCCTCAGAGTGTACAATTACAATCATTTGA20.10432968179447052No Hit
TGGGCATCAGTGATATTGAAGGTACGATTATGTTTATAGGGCTGATAGGT20.10432968179447052No Hit
CTCTTGTTGTGGACAATGGTTCAGGAATGTGCAAAGCCGGCTTCGCCGGT20.10432968179447052No Hit
TGTAAAAAGAATGTAAACCATTGGAAGAAATAGTGTTTATTTTACATATT20.10432968179447052No Hit
GGTTGATAGTGCTTCAGTGACTGGTAACAAACATACTACACGGACAACAT20.10432968179447052No Hit
GGTGACTTGGCCAAGGTCCAGAGAGCCGTGTGCATGTTGAGCAACACAAC20.10432968179447052No Hit
GTTACTATTTCCTACAGTATTATGCTGGTCACATTGGGATGTGTATTACA20.10432968179447052No Hit
GCTGGTGCTTGCTATGCCTGTGTTGGCGGGGGCTATTACAATATTATTAA20.10432968179447052No Hit
CTCCACTCAACCCCAAGGCAAACAGAGAAAAGATGACACAAATCATGTTC20.10432968179447052No Hit
AAAGTGAAGCTGGAATTAATAAACAGATCAACATGGAGCTGTACGCCAGC20.10432968179447052No Hit
CAAAGTCTTTATGATGGACTTCACTTGCAAAGAACTTTGTCAAAGTCTTT20.10432968179447052No Hit
GGTGGTGCTGCCCCAGGTGGCGGTTCCAGTGGTGGCCCAACCATTGAGGA20.10432968179447052No Hit
TGGAACGTTTGATGGTAAAAAAGCATGCTGGGTACCACACGAAAAGGAAG20.10432968179447052No Hit
GGGATATGGGATTGAAAACTGTGGATTTACAGAAGATGTGGGTGTAATGT20.10432968179447052No Hit
CCGGAACCTGTACCTGAACCAGAACCGGAAACTGTTCCTGAACCAGAACC20.10432968179447052No Hit
AAGGCATGGACAATACAGAAAAATACTAAGGCACCTCAGGCTGCTGGTAG20.10432968179447052No Hit
TTAAATAAAAAGGCCGCATTATTGTCCGCTAAAGGCAAGAAGAATCTGCG20.10432968179447052No Hit
TTTGAATACCTATTAGAAGGAAATGTACCTAATGGACACGATTTTAACCG20.10432968179447052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)