FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-7_S55_L007_R2_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-7_S55_L007_R2_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391
Sequences flagged as poor quality0
Sequence length60-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC151.0783608914450036No Hit
CTGACACCCGCTGAAGTGAAGGACTACCTGGCATCTGTACAATGATCTGT50.35945363048166784No Hit
GTTCCTAGCAAGGACTGTCACTGTTATATAAGAGTCCCTAGCAAGGACTG40.28756290438533433No Hit
CTTTCCCTTAATAAAATACCAAATAGTAGTTAAAGAACATAGCAGTAGTA40.28756290438533433No Hit
CCCAGCAACCGTTGCAGAGTTTTACATGTAAATAAGAATGTGAAGATTGA30.2156721782890007No Hit
CGACATTGTGTCTCATTATTATTCGTCTCTCACCACTGATTCAAGTGGGA30.2156721782890007No Hit
GTTACCTGCCCGTCAGTTAATGTGATATTATTGCTAAAGAGCATAAAATA30.2156721782890007No Hit
GGTAACTTATCATTTGACGACATCTTGAAGATTGCCCGCACAATGAGGGA30.2156721782890007No Hit
TAGAAGGATAACATCATTTAGTTTCCTCCTAATCAAGGTTAATGTATGAG30.2156721782890007No Hit
CTTGATTATGATTTTGAGATAATGGGTGTCATTTTTACAAGTCTTTACTA30.2156721782890007No Hit
ATTGAAATGTTTTTGATATGTTATGCTTGTTTGTGAGGTATACACAAGTG30.2156721782890007No Hit
ATTAGATAAAAGTTCATTTGAAGACAGTCCATCCATTTACAGTATTTGCT20.14378145219266716No Hit
ATATCGAGGAAGACCAGAATGATTGATGTTGTATACAATGCTAGTAACAA20.14378145219266716No Hit
CGTTACTGTAAGGACTATCTCTCTCTCTGTCTGTAGGATTTTATTAATAA20.14378145219266716No Hit
GTGTTATTGAAATTTGTGATATTTGAGGATTATTTTTATCAGGAATAAAT20.14378145219266716No Hit
CGGCCGCAGTTAGAAGCTGTGCTAAGGTAGCGTAATAAATTGTCTTTTAA20.14378145219266716No Hit
CTGCTCGTGTTATCATCGAGAAGTATTACACTAAGCTGACCCAAGATTTC20.14378145219266716No Hit
GGCCAATGCCTATATAAAAGTCGAAGGACGTTTCCACTACAAGAAGTGGT20.14378145219266716No Hit
CTTTAACTTACATATTGAAATATCTACGTCTACTGTATTGATGATTGTTG20.14378145219266716No Hit
TTCAAACGTAACGCACACACGCCAGAACTTAATTTTCATCAAAACACAGC20.14378145219266716No Hit
TGGAAATAAACGACTTTCTTATGGAAAAGCTTGTACAGTAAACATCTTAA20.14378145219266716No Hit
ATCATGGAAGGAAAGGTCACTCTCGATTCCTTGAAGATCCATGCTGCTGC20.14378145219266716No Hit
CAGGTCTAAGATCAAGCCTTTCCTCAGAGTGTACAATTACAATCATTTGA20.14378145219266716No Hit
TGGGCATCAGTGATATTGAAGGTACGATTATGTTTATAGGGCTGATAGGT20.14378145219266716No Hit
CTCTTGTTGTGGACAATGGTTCAGGAATGTGCAAAGCCGGCTTCGCCGGT20.14378145219266716No Hit
TTGGAATTTTAATCTCAAATATTATGAAATCAGTAAATAAAAGCAGGATG20.14378145219266716No Hit
TGTGATTCACGACTTTCAGAGGAATGTGACGGTGATAGTGGACAATGATG20.14378145219266716No Hit
TGGAATTGAGCATCAACCAACAAGTTGTATACATTAATAAATCGAGTTCT20.14378145219266716No Hit
TGTAAAAAGAATGTAAACCATTGGAAGAAATAGTGTTTATTTTACATATT20.14378145219266716No Hit
GGTTGATAGTGCTTCAGTGACTGGTAACAAACATACTACACGGACAACAT20.14378145219266716No Hit
AGTCTACGTGTTGGCCATCTTTTGGAAACGGACGAACGAACAGGGCGCAT20.14378145219266716No Hit
GGTGACTTGGCCAAGGTCCAGAGAGCCGTGTGCATGTTGAGCAACACAAC20.14378145219266716No Hit
GTTACTATTTCCTACAGTATTATGCTGGTCACATTGGGATGTGTATTACA20.14378145219266716No Hit
GCTGGTGCTTGCTATGCCTGTGTTGGCGGGGGCTATTACAATATTATTAA20.14378145219266716No Hit
TGTGGATCAGCAAGCAGGAATATGACGAATCCGGCCCATCCATCGTCCAC20.14378145219266716No Hit
CTTTCGTCGGTCGGATAGAAGATCGCATGCCAGTGTTACATATTGTTCTC20.14378145219266716No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCAC20.14378145219266716No Hit
CTCCACTCAACCCCAAGGCAAACAGAGAAAAGATGACACAAATCATGTTC20.14378145219266716No Hit
AAAGTGAAGCTGGAATTAATAAACAGATCAACATGGAGCTGTACGCCAGC20.14378145219266716No Hit
CAGGAATTAAAGTATTTAGTTGAATTGCTACAATCAGAGGAAGGAATGTA20.14378145219266716No Hit
CAAAGTCTTTATGATGGACTTCACTTGCAAAGAACTTTGTCAAAGTCTTT20.14378145219266716No Hit
TCCGCTTTTGAGTGCTGCCATATTGGATTTGCTGTTCTGTCATACATGTG20.14378145219266716No Hit
TGGAACGTTTGATGGTAAAAAAGCATGCTGGGTACCACACGAAAAGGAAG20.14378145219266716No Hit
GGGATATGGGATTGAAAACTGTGGATTTACAGAAGATGTGGGTGTAATGT20.14378145219266716No Hit
CCGGAACCTGTACCTGAACCAGAACCGGAAACTGTTCCTGAACCAGAACC20.14378145219266716No Hit
CTGATATCTATATGTATGTTGTTGTTCAATGCATTGAAGTGCTGCTTCCT20.14378145219266716No Hit
AAGGCATGGACAATACAGAAAAATACTAAGGCACCTCAGGCTGCTGGTAG20.14378145219266716No Hit
TTAAATAAAAAGGCCGCATTATTGTCCGCTAAAGGCAAGAAGAATCTGCG20.14378145219266716No Hit
TTTGAATACCTATTAGAAGGAAATGTACCTAATGGACACGATTTTAACCG20.14378145219266716No Hit
TGAAGATGAAGGGTGTAAATCATTAATGAAGCTGTTCTGTACAAAGTCTC20.14378145219266716No Hit
CTGAAGATATGTTTCAAGTCCCTAGAGAATTAGTAGCCAAAATTATTGGA20.14378145219266716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)