FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-7_S55_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-7_S55_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC221.1476264997391759No Hit
AAGATGGCCTCAATTTCAGTTAATCAAGCTTATGAGAGTTGTGTCAGCAC40.20865936358894105No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40.20865936358894105No Hit
TGCTGATCAGATATTTGAACATTTGTTTGAAAACTCTATTAATTTAATTC40.20865936358894105No Hit
GTGACCTTTACATAACAGTGACAGTCCTTACTATTGACTCTTACATAACA40.20865936358894105No Hit
TTGGGAATCACAACTTCATCGGCTTCCAGGGGAACACTTTCTCCCTCGGC30.1564945226917058No Hit
CTTTACTCATCTGGAACATCATATTGATCAGCGTCAATCCCATCAATCAC30.1564945226917058No Hit
TAGAGTAATAACCTACCGGTATTCATATCAATATTAACAAGTTTTTAAAT30.1564945226917058No Hit
GCATCGTCTGCTCGTATGTCAGACACGTGTCTCACCGCCCCGAAACCAGT30.1564945226917058No Hit
GACATATTAATGGAGCTTCCAAAATTTTACAGGCTTGGTGCAAATGGATC30.1564945226917058No Hit
GTTCTATTTATATATAAATAATTCAAACAAATCTGTCTTTCCGAGTACAG30.1564945226917058No Hit
CACGGTTTGATAATGGATGAAGGAAACGAAGTGAAGTTTCATCTCTGTAT30.1564945226917058No Hit
CTGGTATTCAATATTATGAAAAGTATCTCTAATCAGCTGGACTGGTATTC30.1564945226917058No Hit
GTCATATTCCTGCTTGCTGATCCACATCTGTTGGAAGGTGGACAGAGAAG20.10432968179447052No Hit
CGCATCGTCTGCTCGTATGTCAGACACGTGTCTCACCGCCCCGAAACCAG20.10432968179447052No Hit
AAAGCTCTCTTGGCGTACATCAAGTCGAACTTGTGATCAAGACGAGCCCA20.10432968179447052No Hit
AAACATGATATCTCCATCTTCAACTACGTAATCTCGGCCTTTCTGTTTGT20.10432968179447052No Hit
CTGAACTCTGACCTTGCATATGTAATGTATGTAGAGGATGTTTACCACCA20.10432968179447052No Hit
GGGTTCGTGAAGTTGTGCTATGTTCAATTCCACCTCTGCCTTCTTGTTGA20.10432968179447052No Hit
TTCTTTGCTAGTATAGTCCATCATAAAGACTTAGACAAAGTTCTTTGCTA20.10432968179447052No Hit
TGTGCTACGACATAATATGTGTCATGTAAAATTACATCTAAAGAAGCACT20.10432968179447052No Hit
GTCTTCTCTAGGGCCAGAGCAGACTTCATGGCATCAACACCACAACCCCA20.10432968179447052No Hit
TGTTTGAGAAATGTTTGTGTGCTGGTTTGCACTATGAGAGCATTTCCTAC20.10432968179447052No Hit
AGATGATCCAAGTTATATTCTAGAAGAATCTGAGCATTAGAGATATTTTC20.10432968179447052No Hit
TAGAATTTGGCATAACCTCAGTATATTTTATGTCTAACACTGGCTGTACA20.10432968179447052No Hit
GTTGCATATTTCCCTTCTAAAGCCTCACATCTTCGGAACAATTCAAGTAG20.10432968179447052No Hit
CCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.10432968179447052No Hit
GTCGATTGGCTTGGTTAGCAGACTGATAACCACAGTGACCACGACACAGA20.10432968179447052No Hit
GTTACTACTCCTATTACGACACCTCAAACCCCGCATCAAATTCCCATTAA20.10432968179447052No Hit
GTCATAACAATACATATAGTTCAAGGAAACTTTAAACAAAATACTGAGAT20.10432968179447052No Hit
ACGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.10432968179447052No Hit
CCAAGATGGAGATCTCAGGAACGTAATTATCTCTACGTTCACGCTCTTCC20.10432968179447052No Hit
CCGAAATAGACTGATGCCACGTAAAGAATTGTCGACGTAAGATTTTGTTG20.10432968179447052No Hit
CGAGATACCAGCTTCAAACTCACCAACACCGGCAGCAACAATCAACACAG20.10432968179447052No Hit
TTTGCATTGAGACCACGTGATTCGATCTTACAGCTTTGCTTTTCAAATAA20.10432968179447052No Hit
GGCATATCTAATTTTGCAGAGATTTGCAGCCCCAGGACATCCACAACAAC20.10432968179447052No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.10432968179447052No Hit
CCAGGAACGGCGTCCACCTTTAGACCTAGCGAGGGTAAAGTCCAACAATG20.10432968179447052No Hit
TTTTCCTTTTGGGGGCATAATTTATTTGTTTTGTACAGTTTAAAATCGCA20.10432968179447052No Hit
CAGAGTTTCAGCAAAGAATTACATATGTAATTAATGCCAACAGAATAATC20.10432968179447052No Hit
TGCCCTTTACACTTCCTTCTCTTCGAACTACTTAATTATATTTCCTCGCC20.10432968179447052No Hit
TCAAGATTCTGCCTCGGATGCTCACATTTCCAGTGAATGGACATTTTTTG20.10432968179447052No Hit
ATAAACCTATCAGCCCTATAAACATAATCGTACCTTCAATATCACTGATG20.10432968179447052No Hit
GACAAATTCAATTCTTATACTTAAACCCCTAATAACTCAAAATGGGGCTA20.10432968179447052No Hit
AAAAAATCCAAACTACGTCGACAAAATGTCAATATCAAATAGCCGCCTCC20.10432968179447052No Hit
GCAGCATAATGGGATTCGTACCACTGTCTGAACGGTGTGGCATCAATCTG20.10432968179447052No Hit
CTTTTGTCCGCCAAGAGATTGCACTTGAGCAGAAAGCTCATTGACGCGGT20.10432968179447052No Hit
AAGAGCCCCATCAATAAACAAAGATAAACAAAAAAATCCAAACTACGTCG20.10432968179447052No Hit
CTGGGCTTCATCTCCTACATAGCTGTCCTTCTGTCCCATACCGACCATCA20.10432968179447052No Hit
CGCAAAGTGATTCATGTAAGTCCGAACTAACCCAACTAGTAAAAATAGAG20.10432968179447052No Hit
TAATGAAATTCTATCTATGAATATAAACCACATAAACATATTTTATTTAC20.10432968179447052No Hit
CCTTGCTTAAAGTTTTGTCAAAAAGCTTAAAATGATCACGTGACTCGAGC20.10432968179447052No Hit
GCGACATACATGGCGGGGGCGTTGAAGGTCTCGAACATGATTTGTGTCAT20.10432968179447052No Hit
CGGAAAATTCTGTCTTTCTACGGTATTTGACTCAATAGGATAATTTGCAT20.10432968179447052No Hit
CTTCGAATGTCAAATCATTTTTGCAAAATAAATAAGTTAACAAAGTTAAC20.10432968179447052No Hit
CTCCTGATAAGTTCAGCAAATGTCCTTGGCGGGCTCATGTGCTGTCTGTC20.10432968179447052No Hit
TTTTTATTTACTCTCGTGCTTTGTACATGTAATTTCAGTAAGCAGTTTTC20.10432968179447052No Hit
AAGAAACACCTATTCCAGAATAATAACATACAAGTATGATATTAATTTAA20.10432968179447052No Hit
CATATATGTTGTGTCTCGTGTTTTATTTAGTAATACAAATGTCAGATTTG20.10432968179447052No Hit
AAAAAATCAAATTCTGATATTATACATCTTAAAAATGTGCTATTTAATTA20.10432968179447052No Hit
TACAACTGGGTCACCTGGCATGAAGTTACAGTTGGGCAAAGAATTGCATA20.10432968179447052No Hit
GTACAGCCAACTCATTCCACCGTGGAAGTGAAAATCGACACTTCCTATAG20.10432968179447052No Hit
AGAAAAGGCCGGAGTAGGTGTAGATAAAGCCACTAAAGTATCTGGACCTC20.10432968179447052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)