FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-7_S55_L007_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-7_S55_L007_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391
Sequences flagged as poor quality0
Sequence length60-151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC171.2221423436376708No Hit
TGCTGATCAGATATTTGAACATTTGTTTGAAAACTCTATTAATTTAATTC40.28756290438533433No Hit
GTGACCTTTACATAACAGTGACAGTCCTTACTATTGACTCTTACATAACA40.28756290438533433No Hit
AAGATGGCCTCAATTTCAGTTAATCAAGCTTATGAGAGTTGTGTCAGCAC40.28756290438533433No Hit
GACATATTAATGGAGCTTCCAAAATTTTACAGGCTTGGTGCAAATGGATC30.2156721782890007No Hit
TTGGGAATCACAACTTCATCGGCTTCCAGGGGAACACTTTCTCCCTCGGC30.2156721782890007No Hit
CTTTACTCATCTGGAACATCATATTGATCAGCGTCAATCCCATCAATCAC30.2156721782890007No Hit
TAGAGTAATAACCTACCGGTATTCATATCAATATTAACAAGTTTTTAAAT30.2156721782890007No Hit
GTTCTATTTATATATAAATAATTCAAACAAATCTGTCTTTCCGAGTACAG30.2156721782890007No Hit
CACGGTTTGATAATGGATGAAGGAAACGAAGTGAAGTTTCATCTCTGTAT30.2156721782890007No Hit
CTGGTATTCAATATTATGAAAAGTATCTCTAATCAGCTGGACTGGTATTC30.2156721782890007No Hit
GTCATATTCCTGCTTGCTGATCCACATCTGTTGGAAGGTGGACAGAGAAG20.14378145219266716No Hit
AAAGCTCTCTTGGCGTACATCAAGTCGAACTTGTGATCAAGACGAGCCCA20.14378145219266716No Hit
AAACATGATATCTCCATCTTCAACTACGTAATCTCGGCCTTTCTGTTTGT20.14378145219266716No Hit
GCATCGTCTGCTCGTATGTCAGACACGTGTCTCACCGCCCCGAAACCAGT20.14378145219266716No Hit
TTTTCCTTTTGGGGGCATAATTTATTTGTTTTGTACAGTTTAAAATCGCA20.14378145219266716No Hit
CTGAACTCTGACCTTGCATATGTAATGTATGTAGAGGATGTTTACCACCA20.14378145219266716No Hit
CAGAGTTTCAGCAAAGAATTACATATGTAATTAATGCCAACAGAATAATC20.14378145219266716No Hit
TGCCCTTTACACTTCCTTCTCTTCGAACTACTTAATTATATTTCCTCGCC20.14378145219266716No Hit
GGGTTCGTGAAGTTGTGCTATGTTCAATTCCACCTCTGCCTTCTTGTTGA20.14378145219266716No Hit
TCAAGATTCTGCCTCGGATGCTCACATTTCCAGTGAATGGACATTTTTTG20.14378145219266716No Hit
ATAAACCTATCAGCCCTATAAACATAATCGTACCTTCAATATCACTGATG20.14378145219266716No Hit
TTCTTTGCTAGTATAGTCCATCATAAAGACTTAGACAAAGTTCTTTGCTA20.14378145219266716No Hit
GACAAATTCAATTCTTATACTTAAACCCCTAATAACTCAAAATGGGGCTA20.14378145219266716No Hit
TGTGCTACGACATAATATGTGTCATGTAAAATTACATCTAAAGAAGCACT20.14378145219266716No Hit
GTCTTCTCTAGGGCCAGAGCAGACTTCATGGCATCAACACCACAACCCCA20.14378145219266716No Hit
AAAAAATCCAAACTACGTCGACAAAATGTCAATATCAAATAGCCGCCTCC20.14378145219266716No Hit
TGTTTGAGAAATGTTTGTGTGCTGGTTTGCACTATGAGAGCATTTCCTAC20.14378145219266716No Hit
AGATGATCCAAGTTATATTCTAGAAGAATCTGAGCATTAGAGATATTTTC20.14378145219266716No Hit
TAGAATTTGGCATAACCTCAGTATATTTTATGTCTAACACTGGCTGTACA20.14378145219266716No Hit
GCAGCATAATGGGATTCGTACCACTGTCTGAACGGTGTGGCATCAATCTG20.14378145219266716No Hit
AAGAGCCCCATCAATAAACAAAGATAAACAAAAAAATCCAAACTACGTCG20.14378145219266716No Hit
GTTGCATATTTCCCTTCTAAAGCCTCACATCTTCGGAACAATTCAAGTAG20.14378145219266716No Hit
GTCGATTGGCTTGGTTAGCAGACTGATAACCACAGTGACCACGACACAGA20.14378145219266716No Hit
CTGGGCTTCATCTCCTACATAGCTGTCCTTCTGTCCCATACCGACCATCA20.14378145219266716No Hit
GTTACTACTCCTATTACGACACCTCAAACCCCGCATCAAATTCCCATTAA20.14378145219266716No Hit
CGCAAAGTGATTCATGTAAGTCCGAACTAACCCAACTAGTAAAAATAGAG20.14378145219266716No Hit
GTCATAACAATACATATAGTTCAAGGAAACTTTAAACAAAATACTGAGAT20.14378145219266716No Hit
TAATGAAATTCTATCTATGAATATAAACCACATAAACATATTTTATTTAC20.14378145219266716No Hit
CCTTGCTTAAAGTTTTGTCAAAAAGCTTAAAATGATCACGTGACTCGAGC20.14378145219266716No Hit
CCAAGATGGAGATCTCAGGAACGTAATTATCTCTACGTTCACGCTCTTCC20.14378145219266716No Hit
GCGACATACATGGCGGGGGCGTTGAAGGTCTCGAACATGATTTGTGTCAT20.14378145219266716No Hit
CGGAAAATTCTGTCTTTCTACGGTATTTGACTCAATAGGATAATTTGCAT20.14378145219266716No Hit
CTTCGAATGTCAAATCATTTTTGCAAAATAAATAAGTTAACAAAGTTAAC20.14378145219266716No Hit
CTCCTGATAAGTTCAGCAAATGTCCTTGGCGGGCTCATGTGCTGTCTGTC20.14378145219266716No Hit
CCGAAATAGACTGATGCCACGTAAAGAATTGTCGACGTAAGATTTTGTTG20.14378145219266716No Hit
TTTTTATTTACTCTCGTGCTTTGTACATGTAATTTCAGTAAGCAGTTTTC20.14378145219266716No Hit
AAGAAACACCTATTCCAGAATAATAACATACAAGTATGATATTAATTTAA20.14378145219266716No Hit
CGAGATACCAGCTTCAAACTCACCAACACCGGCAGCAACAATCAACACAG20.14378145219266716No Hit
CATATATGTTGTGTCTCGTGTTTTATTTAGTAATACAAATGTCAGATTTG20.14378145219266716No Hit
TTTGCATTGAGACCACGTGATTCGATCTTACAGCTTTGCTTTTCAAATAA20.14378145219266716No Hit
GGCATATCTAATTTTGCAGAGATTTGCAGCCCCAGGACATCCACAACAAC20.14378145219266716No Hit
AAAAAATCAAATTCTGATATTATACATCTTAAAAATGTGCTATTTAATTA20.14378145219266716No Hit
TACAACTGGGTCACCTGGCATGAAGTTACAGTTGGGCAAAGAATTGCATA20.14378145219266716No Hit
GTACAGCCAACTCATTCCACCGTGGAAGTGAAAATCGACACTTCCTATAG20.14378145219266716No Hit
AGAAAAGGCCGGAGTAGGTGTAGATAAAGCCACTAAAGTATCTGGACCTC20.14378145219266716No Hit
CCAGGAACGGCGTCCACCTTTAGACCTAGCGAGGGTAAAGTCCAACAATG20.14378145219266716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)